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Moving beyond traditional validation, the method's \u201cwhiteness\u201d was quantitatively assessed via the RGB model, balancing analytical excellence, ecological impact, and operational practicality. The redness (analytical) of the strategy is highlighted by a robust RP\u2010HPLC method integrated with LC\u2010MS\/MS for the structural elucidation of degradation products. This was further augmented by in silico toxicological mapping and molecular docking against the human glucocorticoid receptor, providing a comprehensive \u201crisk map\u201d of degradants. The greenness (ecological) was optimized through a design of experiments (DoE) approach and the use of core\u2010shell column technology at a reduced flow rate (0.4\u2009mL\/min), achieving an AGREE score of 0.72 and a favorable GAPI profile. The blueness (practicality) was ensured by streamlining the routine analysis for standard UPLC\u2010PDA\/UV systems, ensuring cost\u2010effective tech\u2010transfer for high\u2010throughput quality control. Ultimately, this integrative framework provides a superior safety diagnostic tool for complex ophthalmic formulations, aligning high\u2010tier pharmaceutical accuracy with environmental and economic consciousness.<\/jats:p>","DOI":"10.1002\/bmc.70590","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:36:18Z","timestamp":1787013378000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Method \u201cWhiteness\u201d in the Stability Analysis of Dexamethasone and PVP\u2010I Ophthalmic Suspensions: Synergizing LC\u2010MS\/MS, In Silico Toxicology, and AGREE\/GAPI Metrics"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1568-585X","authenticated-orcid":false,"given":"Likhit","family":"Mandal","sequence":"first","affiliation":[{"name":"Department of Pharmacy Koneru Lakshmaiah Education Foundation  Guntur Andhra Pradesh India"},{"name":"Department of Analytical Research Hetero Labs Ltd  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8024-9126","authenticated-orcid":false,"given":"Praveen Kumar","family":"Vemuri","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Koneru Lakshmaiah Education Foundation  Guntur Andhra Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3069-5742","authenticated-orcid":false,"given":"Narender","family":"Malothu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Koneru Lakshmaiah Education Foundation  Guntur Andhra Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunil","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Department of Research and Development Hetero Labs Ltd  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408347.2022.2032587"},{"key":"e_1_2_11_3_1","volume-title":"Pharmaceutical Stress Testing: Predicting Drug Degradation","author":"Baertschi S. 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