{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T07:26:40Z","timestamp":1786692400697,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82560886"],"award-info":[{"award-number":["82560886"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Epimedii Folium<\/jats:italic>\n                    has been widely used to treat Alzheimer's disease (\n                    <jats:sc>AD<\/jats:sc>\n                    ) in China. However, the potential active components of\n                    <jats:italic>Epimedii Folium<\/jats:italic>\n                    and its mechanism against\n                    <jats:sc>AD<\/jats:sc>\n                    are still not clear. In this study, an ultra\u2010high performance liquid chromatography coupled to quadrupole Orbitrap high\u2010resolution mass spectrometry (UHPLC\u2010Q\u2010OrbitrapHRMS) method was applied to screen the ingredients of\n                    <jats:italic>Epimedii Folium<\/jats:italic>\n                    . Network pharmacology was utilized to explore the potential active components and pharmacological mechanisms of\n                    <jats:italic>Epimedii Folium<\/jats:italic>\n                    against\n                    <jats:sc>AD<\/jats:sc>\n                    . The binding affinity and conformation of key active ingredients and core targets were performed by molecular docking. Consequently, 72 chemical constituents were identified in\n                    <jats:italic>Epimedii Folium<\/jats:italic>\n                    , including four phenolic acids, two quinones, three 9,10\u2010dihydrophenanthrenes, and 63 flavonoids. Network pharmacology analysis revealed that quercetin, apigenin, kaempferol, and luteolin exhibit favorable pharmacological activities. Molecular docking demonstrated that apigenin\u2013TNF, kaempferol\u2013TNF, kaempferol\u2013TP53, and quercetin\u2010TP53 represent compound\u2013target pairs with strong binding affinities. These findings help elucidate the material basis and underlying mechanisms of\n                    <jats:italic>Epimedii Folium<\/jats:italic>\n                    against\n                    <jats:sc>AD<\/jats:sc>\n                    and offer valuable evidence supporting the further development and clinical application of\n                    <jats:italic>Epimedii Folium<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/bmc.70594","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T14:37:53Z","timestamp":1786545473000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated UHPLC\u2010Q\u2010Orbitrap HRMS and Network Pharmacology Approach for Exploring Potential Active Ingredients and Pharmacological Mechanisms of\n                    <i>Epimedii Folium<\/i>\n                    Against Alzheimer's Disease"],"prefix":"10.1002","volume":"40","author":[{"given":"Mengmo","family":"Chen","sequence":"first","affiliation":[{"name":"School of Pharmaceutical and Life Sciences Jiujiang University  Jiujiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical and Life Sciences Jiujiang University  Jiujiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijuan","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical and Life Sciences Jiujiang University  Jiujiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Lei","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine Jiujiang University  Jiujiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2423-1965","authenticated-orcid":false,"given":"Yun","family":"Ling","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical and Life Sciences Jiujiang University  Jiujiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2016.6860"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2020.113467"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3327"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jval.2024.10.3856"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/2302680"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2016.1239092"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-022-00754-8"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0152371"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26123590"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2024.1359176"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytol.2016.07.029"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2021.105081"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4MD00660G"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(94)00926-K"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5442"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c07046"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.70166"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2017.1405410"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.2174\/156720502110250304104224"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.27183"},{"issue":"4","key":"e_1_2_10_22_1","first-page":"2759","article-title":"Recent Progress Regarding Kaempferol for the Treatment of Various Diseases","volume":"18","author":"Ren J.","year":"2019","journal-title":"Experimental and Therapeutic Medicine"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2017.1405412"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu9040337"},{"key":"e_1_2_10_25_1","first-page":"27","article-title":"Epimedium Improves Cognitive Function in Alzheimer's Disease Mice by Promoting Hippocampal Neural Regeneration","volume":"38","author":"Wang X. 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