{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:07:13Z","timestamp":1789661233289,"version":"4.0.1"},"reference-count":25,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R50CA211241"],"award-info":[{"award-number":["R50CA211241"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01CA266172"],"award-info":[{"award-number":["R01CA266172"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01CA294651"],"award-info":[{"award-number":["R01CA294651"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["TL1TR001858"],"award-info":[{"award-number":["TL1TR001858"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Deoxyuridine (dUrd) is often misincorporated into genomic DNA in cancer patients receiving antimetabolite chemotherapy. This activates base excision repair and recruits ataxia telangiectasia\u2010mutated and Rad3\u2010related (ATR), which coordinates DNA repair with nucleotide metabolism by facilitating ribonucleotide reductase (RNR) activity. Ceralasertib (AZD6738) is the most clinically advanced ATR inhibitor (ATRi) and may abrogate this regulatory pathway and diminish RNR\u2010mediated deoxynucleotide triphosphate synthesis. To further understand ATRi\u2010induced dUrd misincorporation, we developed a highly sensitive LC\u2013MS\/MS method to quantitate: deoxyuridine, uridine, guanosine, adenosine, cytidine, thymidine, deoxyguanosine, deoxyadenosine, and deoxycytidine from digested genomic DNA.<\/jats:p>\n                  <jats:p>Assay application was demonstrated with genomic DNA digested from multiple murine cell lines treated with ceralasertib. Chromatographic separation was achieved with an Inertsil ODS\u20103 (150\u2009\u00d7\u20092.1\u2009mm, 3\u2009\u03bcm) column and a gradient elution program of 0.1% formic acid in water and 0.1% formic acid in methanol over an 18\u2010min run time. Detection was performed on a SCIEX 6500+ tandem mass spectrometer. The method proved to be accurate (90.8%\u2013114.2%) and precise (&lt;\u20097.73% CV) across analytes. Freeze\u2013thaw stability (106.4%\u2013114.3%), stability for 12\u2009months at \u221280\u00b0C (88.7%\u2013113.7%), and stability for 4\u2009h at room temperature (104.1%\u2013114.0%) were acceptable across QCs.<\/jats:p>","DOI":"10.1002\/bmc.70599","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T06:31:26Z","timestamp":1786689086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Resolution and Quantitation of Ribonucleosides and Deoxyribonucleosides in Digested Genomic DNA by UHPLC\u2013MS\/MS"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9093-7459","authenticated-orcid":false,"given":"Joshua J.","family":"Deppas","sequence":"first","affiliation":[{"name":"Cancer Therapeutics Program UPMC Hillman Cancer Center  Pittsburgh Pennsylvania USA"},{"name":"Department of Pharmaceutical Sciences, School of Pharmacy University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9123-7836","authenticated-orcid":false,"given":"Reyna","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4398-6271","authenticated-orcid":false,"given":"Robert A.","family":"Parise","sequence":"additional","affiliation":[{"name":"Cancer Therapeutics Program UPMC Hillman Cancer Center  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1592-2388","authenticated-orcid":false,"given":"Pinakin","family":"Pandya","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9672-0886","authenticated-orcid":false,"given":"Christopher J.","family":"Bakkenist","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"},{"name":"Department of Pharmacology and Chemical Biology, School of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8978-9401","authenticated-orcid":false,"given":"Jan H.","family":"Beumer","sequence":"additional","affiliation":[{"name":"Cancer Therapeutics Program UPMC Hillman Cancer Center  Pittsburgh Pennsylvania USA"},{"name":"Department of Pharmaceutical Sciences, School of Pharmacy University of Pittsburgh  Pittsburgh Pennsylvania USA"},{"name":"Department of Oncology Johns Hopkins University School of Medicine and Johns Hopkins Sidney Kimmel Cancer Center  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"issue":"4","key":"e_1_2_8_2_1","first-page":"247","article-title":"Deoxyuracil in DNA in Health and Disease","volume":"23","author":"Chakraborty J.","year":"2020","journal-title":"Current Opinion in Clinical Nutrition and Metabolic 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