{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:06:30Z","timestamp":1789661190292,"version":"4.0.1"},"reference-count":50,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172901"],"award-info":[{"award-number":["32172901"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This acidic ethanol extraction method was adopted to extract total alkaloids from\n                    <jats:italic>lotus<\/jats:italic>\n                    leaves. Single\u2010factor experiments combined with response surface methodology (RSM) were employed to optimite extraction conditions. The optimal extraction parameters were determined as follows: 55\u2009\u00b0C, 77% ethanol, 0.66% hydrochloric acid, under which the extraction yield of total alkaloids reached 4.14%. D101 macroporous resin was selected for the purification of crude alkaloid extracts. The resin exhibited an adsorption capacity of 8.60\u2009mg\/g and a desorption rate of 43.39%. The optimized purification conditions were as follows: 4\u2009mg\/mL sample solution, 50% ethanol eluent, 1.5 BV\/h flow rate, 30\u2009mL loading\/100\u2009mL elution volume. After purification, total alkaloid purity reached 89.31\u2009\u00b1\u20091.33%. Adsorption obeyed pseudo\u2010second\u2010order kinetics and Temkin isotherm, being spontaneous and endothermic. This provides improved methods for isolating bioactive alkaloids from\n                    <jats:italic>lotus<\/jats:italic>\n                    leaf.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70601","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T11:31:43Z","timestamp":1787657503000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimization of the Extraction of Total Alkaloids From\n                    <i>Lotus<\/i>\n                    Leaves by Acidic Ethanol and Its Purification by Macroporous Resins"],"prefix":"10.1002","volume":"40","author":[{"given":"Xueping","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Agriculture and Biology Liaocheng University  Liaocheng 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