{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:07:01Z","timestamp":1789661221962,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Callicarpa nudiflora<\/jats:italic>\n                    Hook. et Arn. (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. nudiflora<\/jats:italic>\n                    <\/jats:styled-content>\n                    ), a traditional Chinese medicine, is traditionally used to treat bacterial infections, hepatitis, and hemorrhage. However, its chemical constituents and in\u00a0vivo metabolic profile have not yet been systematically elucidated, and comparisons of different extracts are still lacking. This study aimed to systematically characterize and compare the in\u00a0vitro chemical constituents and in\u00a0vivo metabolites of aqueous (CNW) and ethanol (CNE) extracts of\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">C. nudiflora<\/jats:styled-content>\n                    <\/jats:italic>\n                    . A total of 86 chemical constituents were identified in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. nudiflora<\/jats:italic>\n                    <\/jats:styled-content>\n                    using UHPLC\u2010Q\u2010TOF\u2010MS\/MS combined with molecular networking (MN), including 33 flavonoids, 18 phenylethanoid glycosides, 17 iridoids, 9 phenylpropanoids, 8 terpenoids, and 1 other compound. Based on the identified in\u00a0vitro constituents and metabolic rules, 12 prototypes and 53 metabolites were further confirmed and annotated in\u00a0vivo. Furthermore, PCA and OPLS\u2010DA revealed 21 differential in\u00a0vitro constituents and 16 in\u00a0vivo compounds between CNW and CNE. These findings indicated that extraction solvent markedly influences the chemical composition and metabolic behavior of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. nudiflora<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Overall, this study provides a comprehensive and integrated characterization of phytochemicals and in\u00a0vivo metabolic profiles of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. nudiflora<\/jats:italic>\n                    <\/jats:styled-content>\n                    , offering a robust analytical framework for the comparative evaluation and quality control of different extracts.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70604","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:46:17Z","timestamp":1787147177000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Characterization of Phytochemicals and In\u00a0Vivo Metabolites of Aqueous and Ethanol Extracts of\n                    <scp>\n                      <i>Callicarpa nudiflora<\/i>\n                    <\/scp>\n                    by UHPLC\u2010Q\u2010TOF\u2010MS\/MS and Molecular Networking Combined Multivariate Statistical Methods"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2668-7265","authenticated-orcid":false,"given":"Yan","family":"Chen","sequence":"first","affiliation":[{"name":"Research Institute of Marine Traditional Chinese Medicine Shandong University of Traditional Chinese Medicine  Qingdao China"},{"name":"Qingdao Academy of Chinese Medical Sciences Shandong University of Traditional Chinese Medicine  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu Qi","family":"Mei","sequence":"additional","affiliation":[{"name":"College of Pharmacy Binzhou Medical University  Yantai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao Yu","family":"Su","sequence":"additional","affiliation":[{"name":"Research Institute of Marine Traditional Chinese Medicine Shandong University of Traditional Chinese Medicine  Qingdao China"},{"name":"Qingdao Academy of Chinese Medical Sciences Shandong University of Traditional Chinese Medicine  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9343-5332","authenticated-orcid":false,"given":"Jian","family":"Hou","sequence":"additional","affiliation":[{"name":"Research Institute of Marine Traditional Chinese Medicine Shandong University of Traditional Chinese Medicine  Qingdao China"},{"name":"Qingdao Academy of Chinese Medical Sciences Shandong University of Traditional Chinese Medicine  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke Jian","family":"Li","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine Shandong University of Traditional Chinese Medicine  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Hou","sequence":"additional","affiliation":[{"name":"Research Institute of Marine Traditional Chinese Medicine Shandong University of Traditional Chinese Medicine  Qingdao China"},{"name":"Qingdao Academy of Chinese Medical Sciences Shandong University of Traditional Chinese Medicine  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Pharmacopoeia of the People's Republic of China","author":"Chinese Pharmacopoeia Commission","year":"2025"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2025.02.019"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202200444"},{"issue":"4","key":"e_1_2_8_5_1","first-page":"95","article-title":"Research Progress on Chemical Constituents, Pharmacological Effects and Clinical Applications of Callicarpa nudiflora","volume":"43","author":"Kang X. 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