{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:50:22Z","timestamp":1791982222263,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81703683"],"award-info":[{"award-number":["81703683"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Shenyanshu tablets (SYST), a widely prescribed traditional Chinese patent medicine, which is clinically applied to treat chronic glomerulonephritis in China and other Asian countries. However, there is still no detailed report existing on its chemical composition at present, which hinders further investigation into its pharmacological activities and mechanism of action. To solve this difficult problem, an ultra\u2010high\u2010performance liquid chromatography quadrupole time\u2010of\u2010flight mass spectrometry method was established to separate and identify the chemical constituents of SYST. A total of 130 compounds were identified or characterized, among which 19 compounds were clearly confirmed by comparing their retention time and mass spectrometry data with those of reference standards. Based on the mass spectrometry data and relevant literature, the remaining compounds were preliminarily characterized. Results revealed that flavonoids, triterpenoid glycosides, organic acids, and alkaloids were the primary chemical components of SYST. The established analytical method has been proven to effectively characterize the chemical constituents of SYST and also provides important reference for clarifying the pharmacodynamic material basis and mechanism of action of SYST.<\/jats:p>","DOI":"10.1002\/bmc.70606","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:53:04Z","timestamp":1790833984000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of the Chemical Constituents in Shenyanshu Tablets by Ultra\u2010High\u2010Performance Liquid Chromatography Quadrupole Time\u2010of\u2010Flight Mass Spectrometry"],"prefix":"10.1002","volume":"40","author":[{"given":"Xing","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"},{"name":"School of Pharmacy Yanbian University  Yanji Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Guan","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunfu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jilin Luwang Pharmaceutical Co. Ltd  Jilin Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanwu","family":"Guan","sequence":"additional","affiliation":[{"name":"Jilin Luwang Pharmaceutical Co. Ltd  Jilin Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihe","family":"Wang","sequence":"additional","affiliation":[{"name":"Jilin Luwang Pharmaceutical Co. Ltd  Jilin Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"},{"name":"School of Pharmacy Yanbian University  Yanji Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangyu","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3945-8455","authenticated-orcid":false,"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"},{"name":"School of Pharmacy Yanbian University  Yanji Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2999-2344","authenticated-orcid":false,"given":"Lele","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8445-5747","authenticated-orcid":false,"given":"Yue","family":"Cui","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0212-0121","authenticated-orcid":false,"given":"Heyun","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jilin Medical University  Jilin Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2019.14745"},{"key":"e_1_2_10_3_1","first-page":"1669","article-title":"Clinical Study on Shenyanshu Tablets Combined With Mycohenolate Mofetil in Treatment of Chronic Nephritis","volume":"36","author":"Cheng Q.","year":"2021","journal-title":"Drugs and Clinic"},{"key":"e_1_2_10_4_1","first-page":"2070","article-title":"Chemical Variation in Aurantii Fructus Before and After Processing Based on UHPLC\u2010Q\u2010TOF\u2010MS","volume":"6","author":"Cheung T. K.","year":"2016","journal-title":"China Journal of Chinese Materia Medica"},{"key":"e_1_2_10_5_1","volume-title":"Pharmacopoeia of the People's Republic of China","author":"Chinese Pharmacopoeia Commission","year":"2025"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10600-013-0769-8"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202300771"},{"key":"e_1_2_10_8_1","first-page":"306","article-title":"Study on the Metabolic Pathway of Tetrandrine in Rats In\u00a0Vivo","volume":"35","author":"Gu Y.","year":"2012","journal-title":"Journal of Jining Medical University"},{"key":"e_1_2_10_9_1","first-page":"503","article-title":"Effects of Ingredient Study in Traditional Chinese Medicine Research","volume":"34","author":"Guo L.","year":"2009","journal-title":"China Journal of Chinese Materia Medica"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2026.121314"},{"key":"e_1_2_10_11_1","first-page":"1542","article-title":"Chemical Profiling of Raw Product of Semen Cuscuta and Stir\u2010Baking Semen Cuscuta With Salt Solution Based on UHPLC\u2010Q\/TOF\u2010MS Combined Multivariate Statistical Analysis","volume":"31","author":"Huang Y.","year":"2022","journal-title":"Chinese Journal of New Drugs"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1875-5364(23)60392-9"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202300867"},{"key":"e_1_2_10_14_1","first-page":"850","article-title":"Comparison of Chemical Components of Lonicera fragrantissima and Lonicera japonica Based on LC\u2010MS","volume":"46","author":"Jin Y.","year":"2024","journal-title":"Chinese Traditional Patent Medicine"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570178612666150331204147"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15747"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X14500153"},{"key":"e_1_2_10_18_1","first-page":"1563","article-title":"Chemical Constituents of Fangji Fuling Decoction by HPLC\u2010Q\u2010TOF\u2010MS\/MS","volume":"19","author":"Liu Y.","year":"2021","journal-title":"Central South Pharmacy"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.4103\/wjtcm.wjtcm_64_24"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.2515\/therapie\/2011022"},{"key":"e_1_2_10_21_1","first-page":"497","article-title":"Clinical Observation of Shenyanshu Tablets in the Treatment of 60 Cases of Chronic Glomerulonephritis","volume":"41","author":"Ni Q. F.","year":"2012","journal-title":"Shanxi Medical Journal"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3837270"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2011.651176"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-6386(86)80108-1"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/13880209.2023.2233998"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.4973"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1368319"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmaf012"},{"key":"e_1_2_10_29_1","first-page":"6200","article-title":"Network Meta\u2010Analysis of Efficacy of Seven Chinese Patent Medicines in Treatment of Inflammatory Response in Chronic Glomerulonephritis","volume":"48","author":"Shi R. Y.","year":"2023","journal-title":"Zhongguo Zhong Yao Za Zhi\u2009=\u2009Zhongguo Zhongyao Zazhi\u2009=\u2009China Journal of Chinese Materia Medica"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13765-024-00881-y"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-019-0228-y"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2011.12.009"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201701310"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(04)01135-5"},{"key":"e_1_2_10_35_1","first-page":"1238","article-title":"Rapid Identification of Compositions in Polygonatum cyrtonema Hua by UPLC\u2010ESI\u2010QTOF\/MS","volume":"22","author":"Wu X. Y.","year":"2024","journal-title":"Central South Pharmacy"},{"key":"e_1_2_10_36_1","first-page":"107","article-title":"Effects of TCM Therapy on the Progression of Chronic Renal Failure Caused by Primary Glomerulonephritis","volume":"8","author":"Xiong N. N.","year":"1988","journal-title":"Journal of Traditional Chinese Medicine"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00240-024-01533-y"},{"key":"e_1_2_10_38_1","first-page":"1765","article-title":"Identification Technique for In\u00a0Vivo Ingredients of Traditional Chinese Medicines Based on LC\u2010MS Analysis","volume":"37","author":"Yan G.","year":"2012","journal-title":"China Journal of Chinese Materia Medica"},{"key":"e_1_2_10_39_1","first-page":"14","article-title":"New Polyacetylenes From Roots and Rhizomes of Panax ginseng","volume":"52","author":"Yang X. W.","year":"2021","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_10_40_1","first-page":"2001","article-title":"Analysis of Chemical Constituents in Gandou Fumu Decoction by UHPLC\u2010Q\u2010Orbitrap\u2010MS Technology","volume":"39","author":"Yang Y. L.","year":"2024","journal-title":"China Journal of Chinese Materia Medica"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27134225"},{"key":"e_1_2_10_42_1","first-page":"3226","article-title":"Chemical Constituents in Yuhudie Macula\u2010Removing Cream by UPLC\u2010Q\u2010TOF\u2010MS","volume":"21","author":"Zhang Y.","year":"2023","journal-title":"Central South Pharmacy"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2025.107107"},{"key":"e_1_2_10_44_1","first-page":"97","article-title":"Fragmentation Pathway of Ginsenosides in Panax notoginseng Using Electrospray Ionization\u2010Quadrupole\/Time\u2010of\u2010Flight Mass Spectrometer","volume":"38","author":"Zhao J.","year":"2017","journal-title":"Journal of Chinese Mass Spectrometry Society"},{"key":"e_1_2_10_45_1","first-page":"420","article-title":"Characterization and Identification of Chemical Constituents From Schizonepetae Spica Based on UHPLC\u2010Q\u2010TOF\u2010MS\/MS Technique","volume":"49","author":"Zhao Y. M.","year":"2024","journal-title":"China Journal of Chinese Materia Medica"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.1013992"},{"key":"e_1_2_10_47_1","first-page":"321","article-title":"A UPLC\u2010QTOP\u2010MS\/MS Method for the Characterization of Chemical Constituents of Atractylodes lancea (Thunb.) DC. and Atractylodes chinensis (DC.) Koidz","volume":"28","author":"Zhou J.","year":"2022","journal-title":"Pharmaceutical and Clinical Research"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules201219765"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2021.153644"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70606","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.70606","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70606","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:13:58Z","timestamp":1791980038000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70606"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":48,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1002\/bmc.70606"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70606","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2025-10-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70606"}}