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A total of 107 compounds were identified in PCN, of which 36 prototypes were detected in rat serum. Network pharmacology and molecular docking of these absorbed compounds predicted ADORA2A as the core therapeutic target, with 5\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010methylvisammioside, nobiletin,\n                    <jats:italic>sec<\/jats:italic>\n                    \u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glucosylhamaudol, and 9\u2010oxo\u2010ODE showing binding affinity. In an ovalbumin\u2010induced asthmatic rat model, PCN alleviated airway hyperresponsiveness and lung inflammation. Mechanistically, PCN upregulated ADORA2A, elevated cAMP and PKA phosphorylation, and suppressed NLRP3 expression. This UHPLC\u2010Q\u2010Orbitrap HRMS\u2010based serum pharmacochemistry method effectively identified the bioactive components of PCN, and the integrated strategy demonstrates that PCN exerts anti\u2010asthmatic effects through the ADORA2A\/cAMP\/PKA\/NLRP3 axis.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70607","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T23:00:44Z","timestamp":1792450844000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mechanism of Pingchuanning in Attenuating Asthma Based on UHPLC\u2010Q\u2010Orbitrap HRMS and Network Pharmacology Analysis"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8241-7019","authenticated-orcid":false,"given":"Yue","family":"Zhang","sequence":"first","affiliation":[{"name":"Anhui University of Chinese Medicine  Hefei China"},{"name":"School of Traditional Chinese Medicine Anhui University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8019-2419","authenticated-orcid":false,"given":"Yiwen","family":"Ding","sequence":"additional","affiliation":[{"name":"Anhui University of Chinese Medicine  Hefei China"},{"name":"School of Pharmacy Anhui University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7787-2638","authenticated-orcid":false,"given":"Weidong","family":"Chen","sequence":"additional","affiliation":[{"name":"Anhui University of Chinese Medicine  Hefei China"},{"name":"School of Pharmacy Anhui University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanbo","family":"Bi","sequence":"additional","affiliation":[{"name":"Anhui University of Chinese Medicine  Hefei China"},{"name":"School of Pharmacy Anhui University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Anhui University of Chinese Medicine  Hefei China"},{"name":"School of Pharmacy Anhui University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weidong","family":"Ye","sequence":"additional","affiliation":[{"name":"Anhui University of Chinese Medicine  Hefei China"},{"name":"School of Traditional Chinese Medicine Anhui University of Chinese Medicine  Hefei China"},{"name":"Key Laboratory of Xin'an Medicine, Ministry of Education Anhui University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangming","family":"Fang","sequence":"additional","affiliation":[{"name":"Anhui University of Chinese Medicine  Hefei China"},{"name":"School of Traditional Chinese Medicine Anhui University of Chinese Medicine  Hefei China"},{"name":"Key Laboratory of Xin'an Medicine, Ministry of Education Anhui University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2025.118073"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.15054"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.03393-2020"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-019-1260-2"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2014.11.006"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1655205"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-04-217042"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.55.4.3"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2011.06.014"},{"issue":"2","key":"e_1_2_13_11_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0254-6272(13)60014-X","article-title":"Functional Mechanism of Pingchuanning Decoction on Adjustment of Clara Cell Secretory Protein in Airway Remodeling of Asthmatic Rats","volume":"32","author":"Fang X. 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