{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:09:22Z","timestamp":1789661362910,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81874345"],"award-info":[{"award-number":["81874345"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Morindae Officinalis<\/jats:italic>\n                    Radix (MOR), the dried root of\n                    <jats:italic>Morinda officinalis<\/jats:italic>\n                    How, contains abundant saccharides and is used clinically after different processing procedures. However, the anti\u2010inflammatory material basis of MOR and the chemical consequences of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Lycium barbarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    processing remain insufficiently defined. In this study, high\u2010performance liquid chromatography coupled with evaporative light scattering detection (HPLC\u2010ELSD) was developed for fingerprint analysis of 15 batches of raw MOR and the corresponding\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. barbarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010processed MOR (LB\u2010MOR). Seventeen common peaks were resolved, and 11 saccharides were assigned by comparison with reference standards. Anti\u2010inflammatory activity was evaluated in LPS\u2010stimulated RAW 264.7 macrophages by quantifying nitric oxide (NO), TNF\u2010\u03b1, IL\u20101\u03b2, and IL\u20106. Grey relational analysis and partial least squares regression linked the HPLC fingerprints with bioactivity and identified glucose, sucrose, kestose, and nystose as the principal activity\u2010associated saccharides. Subsequent validation showed that these saccharides inhibited inflammatory mediator release, with kestose and nystose showing the strongest effects. Western blotting further indicated that kestose and nystose restored I\u03baB\u03b1 expression and decreased COX\u20102 expression and the p\u2010p65\/p65 ratio, consistent with suppression of sustained NF\u2010\u03baB signaling. This separation\u2010based fingerprint and spectrum\u2010effect workflow identify anti\u2010inflammatory oligosaccharides in MOR and provides candidate quality markers for raw and processed MOR products.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70611","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:20:24Z","timestamp":1788236424000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Anti\u2010Inflammatory Active Components in\n                    <i>Morinda officinalis<\/i>\n                    via Spectroscopic Effect Relationship Analysis and NF\u2010\u03baB Pathway Inhibition"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4844-7698","authenticated-orcid":false,"given":"Kexu","family":"Dong","sequence":"first","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3153-162X","authenticated-orcid":false,"given":"Yuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihang","family":"Peng","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8071-1328","authenticated-orcid":false,"given":"Jing","family":"Lian","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiushi","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuexin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiren Pharmaceutical Co. Ltd.  Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1499-9568","authenticated-orcid":false,"given":"Pengpeng","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1248-6517","authenticated-orcid":false,"given":"Guoshun","family":"Shan","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixiang","family":"Miao","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9939-5814","authenticated-orcid":false,"given":"Yurou","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2182-7018","authenticated-orcid":false,"given":"Fan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6409-4662","authenticated-orcid":false,"given":"Ji","family":"Shi","sequence":"additional","affiliation":[{"name":"School of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2021.104991"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401\u2010020\u201000597\u2010x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nutrit\/nuab115"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011\u2010022\u201001584\u20100"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112104"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2025.113755"},{"key":"e_1_2_11_8_1","volume-title":"StatPearls","author":"Hannoodee S.","year":"2024"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.13914"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2024.114034"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11011\u2010023\u201001223\u20105"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2025.106429"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sigtrans.2017.23"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2012.06.010"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2021.104800"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a000216"},{"key":"e_1_2_11_17_1","first-page":"204","volume-title":"Pharmacopoeia of the People's Republic of China","author":"Pharmacopoeia of the People's Republic of China","year":"2025"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1435274"},{"key":"e_1_2_11_19_1","volume-title":"StatPearls","author":"Stone W. L.","year":"2024"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.8815"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc\u20102025\u2010011716"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111854"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.1026"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2020.03.001"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/10815589241229693"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2022.123380"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.116911"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010020\u201000312\u20106"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/jpp\/rgad096"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.10.028"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664\u2010023\u201000285\u20109"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.104"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2024.111447"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.855964"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70611","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.70611","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70611","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:30:25Z","timestamp":1789659025000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70611"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":33,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/bmc.70611"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70611","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,31]]},"assertion":[{"value":"2026-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70611"}}