{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:09:22Z","timestamp":1789661362939,"version":"4.0.1"},"reference-count":24,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Daphnoretin (DAP) has various pharmacological activities, but its in\u00a0vivo disposition after nanomicellar formulation remains unclear. This study compared the pharmacokinetics and tissue distribution of free DAP and two polymeric nanomicellar formulations, PP\u2010DAP and GA\u2010DAP, following intravenous administration. Plasma DAP concentrations in rats were determined by UPLC\u2013MS\/MS, and DAP concentrations in mouse tissues were determined by LC\u2013MS\/MS. Compared with free DAP, PP\u2010DAP and GA\u2010DAP showed higher systemic exposure, longer apparent elimination half\u2010lives, and lower apparent clearance. The AUC\n                    <jats:sub>0\u2013\u221e<\/jats:sub>\n                    values of DAP, PP\u2010DAP, and GA\u2010DAP were 5474.14, 11,211.04, and 15,019.86\u2009h\u2009\u00b7\u2009ng\/mL, respectively, and the corresponding\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>1\/2<\/jats:sub>\n                    values were 6.84, 9.24, and 9.90\u2009h. DAP was detected in the heart, liver, spleen, lung, and kidney, with the nanomicellar formulations showing altered tissue distribution and GA\u2010DAP exhibiting relatively sustained hepatic distribution. These findings suggest that nanomicellar formulation alters the in\u00a0vivo disposition of DAP. However, free and micelle\u2010associated DAP were not separately quantified, precluding direct characterization of in\u00a0vivo drug release, and the validation range of the tissue quantification method was limited. Further studies are warranted to evaluate the in\u00a0vivo behavior and liver\u2010directed delivery potential of GA\u2010DAP.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70614","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T05:21:01Z","timestamp":1788844861000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Using UPLC\u2013MS\/MS and LC\u2013MS\/MS to Investigate the Pharmacokinetics in SD Rat Plasma and Tissue Distribution in KM Mice of Daphnoretin Nanomicelles"],"prefix":"10.1002","volume":"40","author":[{"given":"Zhi\u2010min","family":"Lv","sequence":"first","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2236-1275","authenticated-orcid":false,"given":"Guo","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"},{"name":"Guizhou Inheritance Base of Traditional Chinese Medicine Processing Technology  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang\u2010ling","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Second Affiliated Hospital of Guizhou University of TCM  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting\u2010ting","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010ping","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi\u2010qiong","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun\u2010li","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke\u2010xin","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010xin","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"},{"name":"Guizhou Inheritance Base of Traditional Chinese Medicine Processing Technology  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Sui","sequence":"additional","affiliation":[{"name":"Department of Chinese Materia Medica Guizhou University of Traditional Chinese Medicine  Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph16071020"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2019.188314"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/d2tb02001g"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2022.114463"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.12.015"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cts.13810"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/17460441.2021.1961741"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajps.2020.07.005"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/08853282241258161"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389201024666230606122116"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906\u2010021\u201003356\u20105"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.19540\/j.cnki.cjcmm.20221224.501"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748\u2010605X\/ac6b73"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2022.123275"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010020\u201000240\u20103"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11419\u2010024\u201000685\u20101"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28134990"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2021.2016749"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2021.12714"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110682"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2020.1813740"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.965131"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/gastro\/goae094"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70614","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.70614","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70614","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:30:30Z","timestamp":1789659030000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70614"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,7]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/bmc.70614"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70614","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,7]]},"assertion":[{"value":"2026-06-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70614"}}