{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:47:32Z","timestamp":1791982052962,"version":"4.4.0"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Huoxue Huayu Gel Plaster (HXHYGP) is a topical traditional Chinese medicine preparation used for oxaliplatin\u2010induced peripheral neuropathy (OIPN), but its locally exposed bioactive components remain unclear. This study developed an integrated strategy combining pharmacodynamic evaluation, UHPLC\u2010HRMS\u2010based tissue pharmacochemistry, untargeted metabolomics, network pharmacology, molecular docking, and qRT\u2010PCR to identify effective components and explore potential mechanisms. In OIPN rats, HXHYGP improved mechanical and cold sensory dysfunction; reduced plantar tissue injury and inflammatory\u2010cell infiltration; increased nerve growth factor levels; decreased TNF\u2010\u03b1, IL\u20106, and IL\u20101\u03b2 levels; and enhanced nerve conduction velocity. A total of 496 constituents were detected in skin tissue, including 20 tissue\u2010migrant constituents attributed to HXHYGP. Untargeted metabolomics identified 53 OIPN\u2010related differential metabolites, of which 42 were significantly reversed after treatment. Correlation analysis and network pharmacology further identified 14 effective components and 98 common targets, with EGFR, PIK3CA, MTOR, RELA, and MAPK14 as key nodes. Molecular docking supported potential component\u2013target interactions, while qRT\u2010PCR confirmed reduced mRNA expression of these targets after HXHYGP treatment. These findings indicate that HXHYGP may alleviate OIPN by modulating the proposed EGFR\/PI3K\/AKT\/mTOR\/NF\u2010\u03baB signaling network and MAPK14\u2010associated inflammation. This strategy provides an analytical basis for identifying pharmacodynamic substances and potential quality\u2010marker candidates in topical traditional Chinese medicine preparations.<\/jats:p>","DOI":"10.1002\/bmc.70626","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:17:01Z","timestamp":1790252221000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["UHPLC\u2013HRMS\u2010Based Tissue Pharmacochemistry and Metabolomics Reveal the Effective Components and Potential Mechanism of Huoxue Huayu Gel Plaster Against Oxaliplatin\u2010Induced Peripheral Neuropathy"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4227-9295","authenticated-orcid":false,"given":"Yu\u2010jiao","family":"Hua","sequence":"first","affiliation":[{"name":"School of Life Sciences and Health Engineering Jiangnan University  Wuxi Jiangsu Province China"},{"name":"School of Chemical &amp; Material Engineering Jiangnan University  Wuxi Jiangsu Province China"},{"name":"Department of Clinical Pharmacy Affiliated Hospital of Jiangnan University  Wuxi Jiangsu Province China"},{"name":"State Key Laboratory of Neurology and Oncology Drug Development  Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2479-7555","authenticated-orcid":false,"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chemical &amp; Material Engineering Jiangnan University  Wuxi Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4918-503X","authenticated-orcid":false,"given":"Yong\u2010juan","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy Affiliated Hospital of Jiangnan University  Wuxi Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing\u2010hua","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Health Engineering Jiangnan University  Wuxi Jiangsu Province China"},{"name":"School of Chemical &amp; Material Engineering Jiangnan University  Wuxi Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23158664"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.3c00084"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-9-53"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2626-6"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.4149\/gpb_2022015"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics3020198"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-024-00929-7"},{"key":"e_1_2_12_9_1","first-page":"3140","article-title":"Tanshinone IIA Ameliorates Oxaliplatin\u2010Induced Neurotoxicity via Mitochondrial Protection and Autophagy Promotion","volume":"11","author":"Cheng W. 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