{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:50:39Z","timestamp":1791982239335,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470429"],"award-info":[{"award-number":["32470429"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Activity\u2010guided separation of medicinal plants requires linking activity signals and chromatographic resolution in complex matrices. In this study, based on a previously established HPLC\u2013DPPH platform, medium\u2010pressure prefractionation and preparative chromatographic purification were applied for the targeted isolation of free radical scavengers from\n                    <jats:italic>Saxifraga tangutica<\/jats:italic>\n                    (\n                    <jats:italic>S. tangutica<\/jats:italic>\n                    ). Medium\u2010pressure prefractionation reduced sample complexity and enriched active fractions, while Click XION chromatography concentrated the target fraction. Subsequent purification on the ReproSil\u2010Pur C18 AQ column resolved this fraction into two baseline\u2010separated active peaks, affording compounds with purities greater than 95%. The isolated compounds were identified as quercetin\u20103\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010\u03b2\u2010D\u2010glucoside\u20107\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010\u03b2\u2010D\u2010glucoside and quercetin\u20103\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010\u03b2\u2010D\u2010galactoside\u20107\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010\u03b2\u2010D\u2010glucoside, a pair of C\u20104\u2033\u2010epimeric quercetin 3,7\u2010di\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glycosides. Both compounds exhibited DPPH radical\u2010scavenging activity, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 68.94\u2009\u00b1\u20092.77 and 75.59\u2009\u00b1\u20092.14\u2009\u03bcM, respectively. DFT calculations, molecular docking, and ADMET prediction further supported the comparative interpretation of their electronic features, antioxidant\u2010related interaction potential, and predicted pharmacokinetic profiles. Overall, this study achieved the activity guided isolation and activity evaluation of a pair of high\u2010purity C\u20104\u2033\u2010epimeric flavonol diglycosides from\n                    <jats:italic>S. tangutica<\/jats:italic>\n                    , providing a practical chromatographic strategy for the targeted characterization of structurally similar bioactive constituents from medicinal plants.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70627","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:28:05Z","timestamp":1790252885000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Online HPLC\u2013DPPH\u2010Guided Isolation and Free Radical\u2010Scavenging Evaluation of C\u20104\u2033\u2010Epimeric Quercetin Diglycosides From\n                    <i>Saxifraga tangutica<\/i>"],"prefix":"10.1002","volume":"40","author":[{"given":"Chuang","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of High Altitude Hypoxia Environment and Life Health, Engineering Research Center of Tibetan Medicine Detection Technology, School of Medicine Xizang Minzu University  Xianyang Shaanxi China"},{"name":"Qinghai Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology Chinese Academy of Sciences  Xining Qinghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurong","family":"Du","sequence":"additional","affiliation":[{"name":"Key Laboratory of High Altitude Hypoxia Environment and Life Health, Engineering Research Center of Tibetan Medicine Detection Technology, School of Medicine Xizang Minzu University  Xianyang Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianbin","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of High 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