{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T11:50:36Z","timestamp":1792410636666,"version":"4.4.0"},"reference-count":64,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Metabolites from probiotic bacteria are gaining wider recognition as bioactive compounds having possible applications in the food, pharmaceutical, and healthcare sectors. Of these,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bacillus coagulans<\/jats:italic>\n                    <\/jats:styled-content>\n                    is one of the spore\u2010producing probiotics recognized for its robustness and physiological benefits; however, there is a lack of information regarding the chemical diversity of these bacteria. This study aims to identify the bioactive compounds produced by\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. coagulans<\/jats:italic>\n                    <\/jats:styled-content>\n                    (MTCC 9872) through the process of gas chromatography\u2013mass spectrometry (GC\u2013MS). Ethyl acetate, benzene, chloroform, hexane, and methanol were used to obtain the extracts of 4\u2010day\u2010old cultures. A total of 32 metabolites have been identified from diverse classes of chemicals including organic acids, hydrocarbons, carbohydrates, amino acid derivatives, phenols, and esters. Glycerol, azelaic acid, deoxyspergualin, and 2,4\u2010di\u2010tert\u2010butylphenol are some of the important chemicals with antimicrobial, antioxidative, anti\u2010inflammatory, immunomodulating, and antitumor activities. The current findings confirm that\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. coagulans<\/jats:italic>\n                    <\/jats:styled-content>\n                    has potential applications in producing functional foods, nutraceuticals, and therapeutic products.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70629","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T11:37:47Z","timestamp":1792409867000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Bioactive Compounds Produced by Probiotic\n                    <scp>\n                      <i>Bacillus coagulans<\/i>\n                    <\/scp>\n                    Using Gas Chromatography\u2013Mass Spectrometry"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8777-0115","authenticated-orcid":false,"given":"Nidhi","family":"Prajapati","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Smt. S. S. Patel Nootan Science and Commerce College Sankalchand Patel University  Visnagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4792-7100","authenticated-orcid":false,"given":"Arati","family":"Chaudhary","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Smt. S. S. Patel Nootan Science and Commerce College Sankalchand Patel University  Visnagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anamika","family":"Pathak","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Smt. S. S. Patel Nootan Science and Commerce College Sankalchand Patel University  Visnagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinil","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Smt. S. S. Patel Nootan Science and Commerce College Sankalchand Patel University  Visnagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ansh","family":"Prajapati","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Smt. S. S. Patel Nootan Science and Commerce College Sankalchand Patel University  Visnagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4401-8483","authenticated-orcid":false,"given":"Anil","family":"Patani","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Smt. S. S. Patel Nootan Science and Commerce College Sankalchand Patel University  Visnagar India"},{"name":"Gujarat Biotechnology Research Center (GBRC), M.S. Building  Gandhinagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4612-1724","authenticated-orcid":false,"given":"Ashish","family":"Patel","sequence":"additional","affiliation":[{"name":"Gujarat Biotechnology Research Center (GBRC), M.S. Building  Gandhinagar India"},{"name":"Department of Life Sciences Hemchandracharya North Gujarat University  Patan India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7992-3080","authenticated-orcid":false,"given":"Dharmendra","family":"Prajapati","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Smt. S. S. Patel Nootan Science and Commerce College Sankalchand Patel University  Visnagar India"},{"name":"Center for Research and Innovation Sankalchand Patel University  Visnagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"issue":"1","key":"e_1_2_12_2_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s13065-017-0267-3","article-title":"Synthesis, Characterization and Evaluation of 1, 3, 5\u2010Triazine Aminobenzoic Acid Derivatives for Their Antimicrobial Activity","volume":"11","author":"Al\u2010Zaydi K. M.","year":"2017","journal-title":"Chemistry Central Journal"},{"issue":"8","key":"e_1_2_12_3_1","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1111\/j.1525-1594.1996.tb04555.x","article-title":"15\u2010Deoxyspergualin: A Newly Developed Immunosuppressive Agent and Its Mechanism of Action and Clinical Effect: A Review","volume":"20","author":"Amemiya H.","year":"1996","journal-title":"Artificial Organs"},{"key":"e_1_2_12_4_1","unstructured":"Araya M. L.Morelli G.Reid et\u00a0al.2002. \u201cGuidelines for the Evaluation of Probiotics in Food.\u201d Joint FAO\/WHO Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food 1\u201311."},{"issue":"10","key":"e_1_2_12_5_1","doi-asserted-by":"crossref","DOI":"10.1002\/elsc.202300210","article-title":"Bioprocess Development for Endospore Production by Bacillus coagulans Using an Optimized Chemically Defined Medium","volume":"23","author":"Biermann R.","year":"2023","journal-title":"Engineering in Life Sciences"},{"key":"e_1_2_12_6_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2023.464376","article-title":"Use and Abuse of Retention Indices in Gas Chromatography","volume":"1708","author":"Bizzo H. 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