{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:49:51Z","timestamp":1791982191909,"version":"4.4.0"},"reference-count":72,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Medicinal plants have served as a cornerstone of traditional healthcare systems worldwide.\n                    <jats:italic>Pilea scripta<\/jats:italic>\n                    (Buch.\u2010Ham. ex D.Don) Wedd. is a plant traditionally used by indigenous communities for diabetes management, although its phytochemical composition and pharmacological potential remain largely unexplored. This study characterised the phytoconstituents and evaluated the antioxidant and antidiabetic potential of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. scripta<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Preliminary phytochemical screening was performed for the methanolic extract, while total phenolic and flavonoid contents were determined for the methanolic extract and its fractions. Antioxidant and antidiabetic activities were evaluated using DPPH, ABTS, phosphomolybdate, reducing power, DNA strand scission protection, \u03b1\u2010amylase and \u03b1\u2010glucosidase inhibition assays. Metabolite profiling by GC\u2013MS and LC\u2013QTOF\u2010MS was followed by molecular docking of all identified compounds and molecular dynamics simulations of the most potent compounds. Ethyl acetate fraction showed the highest total phenolic (353.61\u2009\u03bcg GAE\/mg extract) and flavonoid (372.53\u2009\u03bcg RE\/mg extract) contents, significant DPPH (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 85.406\u2009\u03bcg\/mL) and ABTS (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 116.25\u2009\u03bcg\/mL) activities, high phosphomolybdate activity, reducing power, DNA strand protection, and potent \u03b1\u2010amylase (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 186.38\u2009\u03bcg\/mL) and \u03b1\u2010glucosidase (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 150.84\u2009\u03bcg\/mL) inhibitory activities. Molecular dynamics simulations confirmed stable interactions of the most potent compounds with both enzymes. These findings support traditional use of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. scripta<\/jats:italic>\n                    <\/jats:styled-content>\n                    for diabetes management.\n                  <\/jats:p>","DOI":"10.1002\/bmc.70634","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:49:51Z","timestamp":1791334191000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Profiling, Antioxidant and Antidiabetic Potential of the Ethyl Acetate Fraction of\n                    <i>Pilea scripta<\/i>\n                    (Buch.\u2010Ham. ex D.Don) Wedd.: Integrated In\u00a0Vitro and In Silico Study"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0813-682X","authenticated-orcid":false,"given":"Navdeep","family":"Kour","sequence":"first","affiliation":[{"name":"Department of Botanical and Environmental Sciences Guru Nanak Dev University  Amritsar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simran","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Botanical and Environmental Sciences Guru Nanak Dev University  Amritsar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7474-7068","authenticated-orcid":false,"given":"Karanvir","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences Guru Nanak Dev University  Amritsar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balbir","family":"Singh","sequence":"additional","affiliation":[{"name":"Department 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