{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:50:39Z","timestamp":1791982239298,"version":"4.4.0"},"reference-count":58,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Heart failure (HF) is an end stage of cardiovascular diseases. Tongmai Qiangshen Medicinal Liquor (TMQSJ) is an over\u2010the\u2010counter traditional Chinese medicinal liquor, but its efficacy\u2010related material basis and mechanisms in HF remain unclear. In this study, UPLC\u2010Q\u2010TOF\u2010MS\/MS was used to characterize TMQSJ constituents and prototype compounds in rat plasma. HPLC fingerprinting, spectrum\u2010effect relationship analysis, single\u2010compound activity evaluation, and integrative bioinformatics were combined to screen efficacy\u2010related candidate quality markers and HF\u2010associated candidate genes. A total of 223 compounds were characterized, including 33 prototype plasma components. Chemometric analysis identified caffeic acid, Epimedin A, Epimedin B, Epimedin C, and icariin as characteristic constituents. Spectrum\u2010effect analysis based on 10 batches of TMQSJ and validation in DOX\u2010injured AC16 cells highlighted Epimedin A, Epimedin B, and Epimedin C. Among them, Epimedin A and Epimedin C showed prototype plasma exposure and were prioritized as efficacy\u2010related candidate quality markers. Integration of predicted targets of prototype plasma components with HF transcriptomic data and machine\u2010learning analysis identified BCL2L1, STAT3, HIF1A, MAPK8, and ESR1 as five candidate core genes. Immune cell estimation and molecular docking provided computational clues. Overall, this study establishes an integrated framework linking chemical quality, pharmacological association, in\u00a0vivo exposure, and mechanistic prediction for TMQSJ quality evaluation.<\/jats:p>","DOI":"10.1002\/bmc.70637","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:55:25Z","timestamp":1791248125000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Quality Markers for Tongmai Qiangshen Medicinal Liquor in the Treatment of Heart Failure Based on Multicomponent Qualitative\u2013Quantitative Analysis and Integrated Bioinformatics"],"prefix":"10.1002","volume":"40","author":[{"given":"Yuanyuan","family":"Li","sequence":"first","affiliation":[{"name":"College of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6916-4081","authenticated-orcid":false,"given":"Jinnan","family":"Huo","sequence":"additional","affiliation":[{"name":"College of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianjiao","family":"Li","sequence":"additional","affiliation":[{"name":"College of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"},{"name":"Professional Innovation Technology Center for Multidimensional Analysis of Traditional Chinese Medicine  Dalian Liaoning China"},{"name":"Liaoning Province Modern Chinese Medicine Research Engineering Laboratory  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8895-5199","authenticated-orcid":false,"given":"Shuai","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"},{"name":"Professional Innovation Technology Center for Multidimensional Analysis of Traditional Chinese Medicine  Dalian Liaoning China"},{"name":"Liaoning Province Modern Chinese Medicine Research Engineering Laboratory  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0588-2113","authenticated-orcid":false,"given":"Yongrui","family":"Bao","sequence":"additional","affiliation":[{"name":"College of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"},{"name":"Professional Innovation Technology Center for Multidimensional Analysis of Traditional Chinese Medicine  Dalian Liaoning China"},{"name":"Liaoning Province Modern Chinese Medicine Research Engineering Laboratory  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2814-4615","authenticated-orcid":false,"given":"Xiansheng","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian Liaoning China"},{"name":"Professional Innovation Technology Center for Multidimensional Analysis of Traditional Chinese Medicine  Dalian Liaoning China"},{"name":"Liaoning Province Modern Chinese Medicine Research Engineering Laboratory  Dalian Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sapharm.2025.07.005"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011\u2010023\u201001845\u20106"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2024.07.001"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.988360"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2013.12.028"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2004.05.011"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13148\u2010019\u20100730\u20101"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.109.158642"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehv087"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.8b00312"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.968373"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1150304"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00229.2006"},{"key":"e_1_2_11_16_1","first-page":"7679","article-title":"Analysis of Components in Qidong Yixin Oral Liquid by HPLC Fingerprint and UPLC\u2010Q\u2010TOF\u2010MS","volume":"53","author":"Han M. X.","year":"2022","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2016.163"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.34133\/research.1224"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.4c05652"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2024.1463666"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202300677"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1691\/ph.2024.3647"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20105922.12121"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10010051"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u20109\u2010559"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.112.000207"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.31083\/j.fbl2703101"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3007"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2025.157135"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020\u2010021\u201000508\u20100"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2023.10.004"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4261"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2014.12.002"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1875\u20105364(19)30086\u2010X"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/jnme\/6614635"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3337"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033\u2010026\u201011818\u20107"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2026.117753"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2026.103016"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2013.05.028"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010023\u201001660\u20109"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1560903"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.989286"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2021.05.013"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100141"},{"key":"e_1_2_11_47_1","first-page":"106","article-title":"Exploring the Construction of Healthcare Wine Culture From the Perspective of Traditional Chinese Medicine Theory and Modernization of Chinese Materia Medica","volume":"11","author":"Xie X.","year":"2021","journal-title":"Liquor\u2010Making Science & Technology"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12872\u2010025\u201005442\u20109"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020\u2010024\u201001018\u20105"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.3413"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.990888"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2025.120899"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2026.117491"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1055\/a\u20102186\u20103182"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0AY01639J"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2025.102770"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.70460"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.13562"},{"key":"e_1_2_11_59_1","first-page":"649","article-title":"Components and Mechanism of Cuscutae Semen\u2010Lycii Fructus Herb Pair in Treatment of Premature Ovarian Insufficiency Based on UPLC\u2010Q\u2010TOF\u2010MS and Network Pharmacology","volume":"47","author":"Zong Z.","year":"2025","journal-title":"Chinese Traditional Patent Medicine"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70637","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.70637","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.70637","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:09:16Z","timestamp":1791979756000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.70637"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,5]]},"references-count":58,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1002\/bmc.70637"],"URL":"https:\/\/doi.org\/10.1002\/bmc.70637","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,5]]},"assertion":[{"value":"2026-06-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70637"}}