{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T01:39:14Z","timestamp":1789436354539,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,11,2]],"date-time":"2006-11-02T00:00:00Z","timestamp":1162425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple, high\u2010throughput, highly selective and sensitive HPLC\u2010FLD method for isolation and determination of furosemide and\/or norfloxacin in human plasma samples following a simple organic solvent deproteinization step with acetonitrile as sample \u2018clean\u2010up\u2019 procedure is reported. One of the two drug substances plays the internal standard role for the determination of the other. Separation of analyte and internal standard was achieved in less than 5.3 min (injection to injection) on a Chromolith Performance RP\u201018e column, using an aqueous component containing 0.015 mol\/L sodium heptane\u2010sulfonate and 0.2% triethylamine brought to pH = 2.5 with H<jats:sub>3<\/jats:sub>PO<jats:sub>4<\/jats:sub>. The composition of the mobile phase was: acetonitrile\u2013methanol\u2013aqueous component = 70:15:15 (v\/v\/v) and the flow\u2010rate was set up to 3 mL\/min. The chromatographic method applied to the determination of furosemide relies on fluorescent detection parameters of 235 nm for the excitation wavelength, and 402 nm for the emission wavelength. In case of norfloxacin, the excitation wavelength is set up to 268 nm and the emission wavelength is set up to 445 nm. The overall method leads to quantitation limits of about 27 ng\/mL for furosemide, and 19.5 ng\/mL for norfloxacin, using an injection volume of 250 \u00b5L. The method was applied to the bioequivalence study of two furosemide\u2010containing formulations. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.715","type":"journal-article","created":{"date-parts":[[2006,11,2]],"date-time":"2006-11-02T07:01:45Z","timestamp":1162450905000},"page":"40-47","source":"Crossref","is-referenced-by-count":18,"title":["High\u2010throughput liquid\u2010chromatography method with fluorescence detection for reciprocal determination of furosemide or norfloxacin in human plasma"],"prefix":"10.1002","volume":"21","author":[{"given":"Toma","family":"Galaon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Udrescu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iulia","family":"Sora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"David","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei","family":"Medvedovici","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,11,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-827X(00)00064-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00058-9"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.2460\/ajvr.1992.53.11.2084","article-title":"Pharmacokinetics of norfloxacin and its N\u2010desethyl\u2010 and oxo\u2010metabolites in broiler chickens","volume":"53","author":"Anadon A","year":"1992","journal-title":"American Journal of Veterinary Research"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000117773.22969.B3"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826079708005549"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600810621"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(96)00706-6"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0717-97072005000200008"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(97)00055-8"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00066-1"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2004.04.059"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600840120"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00176-0"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(96)00096-5"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(92)80155-J"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(93)80380-M"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1130030607"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/43.12.2274"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(91)90812-5"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2004.09.034"},{"key":"e_1_2_1_22_1","first-page":"115","article-title":"Sample preparation and RPHPLC determination of diuretics in human body fluids","volume":"56","author":"Zendelovska D","year":"2006","journal-title":"Acta Pharmaceutica"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.715","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.715","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:57:33Z","timestamp":1760050653000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.715"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11,2]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1002\/bmc.715"],"URL":"https:\/\/doi.org\/10.1002\/bmc.715","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11,2]]}}}