{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T21:58:26Z","timestamp":1785189506974,"version":"3.55.0"},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,1,17]],"date-time":"2007-01-17T00:00:00Z","timestamp":1168992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Four major triterpenoids (ganoderic acids C<jats:sub>2<\/jats:sub>, B, K and H) in rat plasma after oral administration of <jats:italic>G. lucidum<\/jats:italic> extract were analyzed quantitatively by high\u2010performance liquid chromatography (HPLC). Plasma samples taken from rats were acidified with hydrochloric acid and extracted with dichloromethane\u2013ethyl acetate (90:10). The chromatographic separation was achieved on an Agilent Zorbax SB\u2010C<jats:sub>18<\/jats:sub> column (250 \u00d7 4.6 mm, 5 \u00b5m) at 35\u00b0C, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v\/v), at a flow rate of 1.0 mL\/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. All calibration curves showed good linearity (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup> &gt; 0.99) within test ranges. The relative deviation of this method was less than 10% for intra\u2010 and inter\u2010day assays, and the accuracy ranged from 89 to 108%. The extract recovery for the four triterpenoids and internal standard ranged from 95 to 67%, and the QC samples were found to be stable according to the results of the stability study. This is the first report on determination of the major triterpenoids in rat plasma after oral administration of <jats:italic>G. lucidum<\/jats:italic> extract and the results provided a firm basis for clarifying the pharmacological effect of <jats:italic>G. lucidum<\/jats:italic> and evaluating the clinical applications of this medicinal fungus. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.768","type":"journal-article","created":{"date-parts":[[2007,1,17]],"date-time":"2007-01-17T12:19:01Z","timestamp":1169036341000},"page":"389-396","source":"Crossref","is-referenced-by-count":23,"title":["HPLC method for the determination and pharmacokinetic studies of four triterpenoids in rat plasma after oral administration of <i>Ganoderma lucidum<\/i> extract"],"prefix":"10.1002","volume":"21","author":[{"given":"Xiaoming","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongxia","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianghao","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhong","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaishun","family":"Bi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,1,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"940","article-title":"Development of triterpenes from Ganoderma lucidum","volume":"25","author":"Chen RY","year":"1990","journal-title":"Acta Pharmaceutica Sinica"},{"key":"e_1_2_1_3_1","unstructured":"FDA.Guidance for Industry Bioanalytical Method Validation. Food and Drug Administration Centre for Drug Evaluation and Research May 2001;www.fda.gov\/cder\/guidance\/4252fnl.htm"},{"key":"e_1_2_1_4_1","first-page":"154","article-title":"Enzyme immunoassay for the quantitative determination of ganoderic acid A from Ganoderma lucidum","volume":"18","author":"Gao JJ","year":"2001","journal-title":"Journal of Traditional Medicine"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1506"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.34.3695"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","unstructured":"KohdaH TokumotoW SakamotoK FujiiM HiraiY YamasakiK KomodaY NakamuraH IshiharaSandUchidaM.The biologically active constituents ofGanoderma lucidum(FR.) Karst. Histamine release\u2010inhibitory triterpenes.Chemical & Pharmaceutical Bulletin1985;33:1367\u20131374.","DOI":"10.1248\/cpb.33.1367"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.19820650221"},{"key":"e_1_2_1_9_1","first-page":"7","volume-title":"Modern Research of Ganoderma lucidum","author":"Lin ZB","year":"2001"},{"key":"e_1_2_1_10_1","first-page":"395","article-title":"Difficulty and hot\u2010points on pharmacokinerics studies of traditional Chinese medicine","volume":"40","author":"Liu CX","year":"2005","journal-title":"Acta Pharmaceutica Sinica"},{"key":"e_1_2_1_11_1","first-page":"574","article-title":"Advances of pharmacological effects of triterpenes from Ganoderma lucidum","volume":"37","author":"Luo J","year":"2002","journal-title":"Acta Pharmaceutica Sinica"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.34.3025"},{"key":"e_1_2_1_13_1","first-page":"113","article-title":"Synergic effect China ganoderma lucidum essence powder has in enhancing the efficacy of chemotherapy and in fighting against side effects of chemicals","volume":"4","author":"Ren W","year":"2004","journal-title":"Chinese Journal of Medicine"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2005.12.015"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X05003429"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2006.01.053"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.768","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.768","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:54:30Z","timestamp":1760050470000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.768"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1,17]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1002\/bmc.768"],"URL":"https:\/\/doi.org\/10.1002\/bmc.768","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1,17]]}}}