{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:49:20Z","timestamp":1760060960618,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2007,6,4]],"date-time":"2007-06-04T00:00:00Z","timestamp":1180915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>During a study of 100 \u00b5L aliquots of urocortin containing various acetonitrile contents, we hypothesized that a change in the acetonitrile content in the solution across a specific content of acetonitrile (critical threshold) causes an abrupt change in adsorption capacity to the column packing. Circular dichroism measurements suggest that the conformational change induced by acetonitrile in the solution causes the abrupt change in adsorption capacity, and this solvent\u2010induced conformational change is reversible across the critical threshold. This hypothesis can apply to various polypeptides with molecular weights range from 1007 to 6789 and to other organic solvents. A new gradient system utilizing the instant recovery of the adsorption capacity across the critical threshold was designed, and applied to the analysis of a 100 \u00b5L aliquot of various polypeptide solutions. The results suggest that use of a solution containing organic solvents more than the critical threshold allows successful dilution of polypeptides up to picomolar concentration range without any loss due to its adsorption during handling procedures and loading onto the LC system, and that a new gradient system enables quantitative analysis of polypeptides at picomolar concentrations in such solutions. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.825","type":"journal-article","created":{"date-parts":[[2007,6,4]],"date-time":"2007-06-04T11:46:35Z","timestamp":1180957595000},"page":"1005-1015","source":"Crossref","is-referenced-by-count":5,"title":["Highly sensitive and quantitative analysis of polyeptides using a new gradient system based on an abrupt change in adsorption of polypeptide to the reversed\u2010phase column packing"],"prefix":"10.1002","volume":"21","author":[{"given":"Ryoya","family":"Goda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichi","family":"Sudo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2004.08.016"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2675315"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/13690"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1546328"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(01)00068-9"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(00)00168-9"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac030163g"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0494376"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-003-2298-y"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac960760l"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)96966-8"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.04.005"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M200025-MCP200"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9888(1998100)33:10<976::AID-JMS710>3.0.CO;2-7"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00038a021"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.063081"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00316-1"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.10.036"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr0503681"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0231(20000930)14:18<1677::AID-RCM84>3.0.CO;2-N"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00094a011"},{"key":"e_1_2_1_23_1","first-page":"6541","article-title":"High sequence coverage of proteins isolated from liquid separations of breast cancer cells using capillary electrophoresis\u2010time\u2010of\u2010flight MS and MALDI\u2010TOF MS mapping","volume":"76","author":"Zhu K","year":"2003","journal-title":"Analytical Chemistry"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.825","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.825","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:24:10Z","timestamp":1760037850000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.825"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,4]]},"references-count":22,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1002\/bmc.825"],"URL":"https:\/\/doi.org\/10.1002\/bmc.825","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2007,6,4]]}}}