{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:24:01Z","timestamp":1777454641321,"version":"3.51.4"},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2007,5,2]],"date-time":"2007-05-02T00:00:00Z","timestamp":1178064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Simultaneous determination of 3,4\u2010methylenedioxymethamphetamine (MDMA) and 3,4\u2010methylenedioxyamphetamine (MDA) in rat blood and brain microdialysates by high\u2010performance liquid chromatography with fluorescence detection (HPLC\u2010FL) was developed. Microdialysates were directly subjected to derivatization with 4\u2010(4,5\u2010diphenyl\u20101<jats:italic>H<\/jats:italic>\u2010imidazol\u20102\u2010yl)benzoyl chloride (DIB\u2010Cl). The DIB\u2010derivatives of MDMA, MDA and the internal standard, 1\u2010methyl\u20103\u2010phenylpropylamine (MPPA), were isocratically separated on an ODS column using a mixture of 50 m<jats:sc>m<\/jats:sc> phosphate buffer (pH 7.0)\u2013acetonitrile\u2013methanol\u20132\u2010propanol (50:45:5:2, v\/v\/v\/v %) as an eluent at a flow rate of 1.5 mL\/min. The calibration curves of MDA and MDMA spiked to blood and brain microdialysates were linear over the ranges 2.5\u2013500 and 5.0\u20131000 ng\/mL, respectively. The detection limits of MDA and MDMA were 1.2 and 4.2 for blood and 1.3 and 4.8 ng\/mL for brain, respectively. Additionally, the intra\u2010 and the inter\u2010assay precisions were lower than 5.6% for the blood and brain microdialysates (<jats:italic>n<\/jats:italic> = 4). The proposed method was successfully applied for the monitoring of MDMA and its metabolite MDA in rat blood and brain microdialysates, and the pharmacokinetic parameters of MDMA and MDA in the microdialysates after administration of MDMA (5 mg\/kg, i.p.) with or without caffeine (20 mg\/kg, i.p.) were evaluated. 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