{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:58:38Z","timestamp":1785459518153,"version":"3.56.0"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2007,4,20]],"date-time":"2007-04-20T00:00:00Z","timestamp":1177027200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A rapid and sensitive reversed\u2010phase high\u2010performance liquid chromatographic method (RP\u2010HPLC) with ultraviolet detection has been developed for the determination of moclobemide and its metabolites, <jats:italic>p<\/jats:italic>\u2010chloro\u2010<jats:italic>N<\/jats:italic>\u2010(\u20102\u2010morpholinoethyl)benzamide <jats:italic>N<\/jats:italic>\u2032\u2010oxide (Ro 12\u20105637) and <jats:italic>p<\/jats:italic>\u2010chloro\u2010<jats:italic>N<\/jats:italic>\u2010[2\u2010(3\u2010oxomorpholino)ethyl]\u2010benzamide (Ro 12\u20108095), in human plasma. The assay was performed after single liquid\u2013liquid extraction with dichloromethane at alkaline pH using phenacetin as the internal standard. Chromatographic separation was performed on a C<jats:sub>18<\/jats:sub> column using a mixture of acetonitrile and water (25:75, v\/v), adjusted to pH 2.7 with <jats:italic>ortho<\/jats:italic>\u2010phosphoric acid, as mobile phase. Spectrophotometric detection was performed at 239 nm. The method has been validated for accuracy, precision, selectivity, linearity, recovery and stability. The quantification limit for moclobemide and Ro 12\u20108095 was 10 ng\/mL, and for Ro 12\u20105637 was 30 ng\/mL. Linearity of the method was confirmed for the range 20\u20132500 ng\/mL for moclobemide (<jats:italic>r<\/jats:italic> = 0.9998), 20\u20131750 ng\/mL for Ro 12\u20108095 (<jats:italic>r<\/jats:italic> = 0.9996) and 30\u2013350 ng\/mL for Ro 12\u20105637 (<jats:italic>r<\/jats:italic> = 0.9991). Moreover, within\u2010day and between\u2010day precisions and accuracies of the method were established. The described method was successfully applied in pharmacokinetic studies of parent drug and its two metabolites after a single oral administration of 150 mg of moclobemide to 20 healthy volunteers. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.840","type":"journal-article","created":{"date-parts":[[2007,4,20]],"date-time":"2007-04-20T13:48:33Z","timestamp":1177076913000},"page":"958-966","source":"Crossref","is-referenced-by-count":10,"title":["A validated high\u2010performance liquid chromatographic method for the determination of moclobemide and its two metabolites in human plasma and application to pharmacokinetic studies"],"prefix":"10.1002","volume":"21","author":[{"given":"Alina","family":"Plenis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Chmielewska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucyna","family":"Konieczna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henryk","family":"Lamparczyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,4,20]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(97)00086-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.04.016"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1111\/j.1527-3458.2003.tb00245.x","article-title":"Moclobemide: therapeutic use and clinical studies","volume":"1","author":"Bonnet U","year":"2003","journal-title":"CNS Drug Reviews"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199652030-00013"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0379-0738(97)00075-3"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(87)80160-3"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-199502000-00007"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-9236(95)90230-9"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0447.1990.tb05345.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0924-977X(96)00023-5"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00625-3"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/00498250110055488"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1081\/JLC-100103390"},{"key":"e_1_2_1_15_1","first-page":"3","article-title":"Studies on rapid analysis of toxicants and drugs in body fluids VII. 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