{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:55:21Z","timestamp":1760061321002,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2001,9,4]],"date-time":"2001-09-04T00:00:00Z","timestamp":999561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A high performance liquid chromatographic method has been developed for the simultaneous determination of (\u00b1) fenfluramine (Fen) and phentermine (Phen) in addition to three other sympathomimetic amines\u2014ephedrine (E), norephedrine (NE) and 2\u2010phenylethylamine (2\u2010PEA), using cyclohexylamine (CX) as an internal standard in plasma. The compounds were derivatized with 4\u2010(4,5\u2010diphenyl\u20101<jats:italic>H<\/jats:italic>\u2010imidazole\u20102\u2010yl)benzoyl chloride (DIB\u2010Cl) to give the DIB\u2010derivatives. The derivatives were then separated using an isocratic HPLC system with UV detection. The limits of detection for Fen, Phen, E, NE and 2\u2010PEA in plasma ranged from 0.32 to 22.9\u2005pmol on column at a signal\u2010to\u2010noise ratio of 3. The recoveries following alkaline extraction from plasma samples of known concentrations were found to be more than 94% for the studied compounds. This method might be useful for the screening of the studied sympathomimetic amines in human plasma samples in forensic as well as toxicological studies. Furthermore, the developed method was modified for the simultaneous determination of Fen and Phen in human and rat plasma using fluoxetine as an internal standard. The methods are reproducible and precise. Finally, the two drugs were administered intraperitonealy to rats in combination, and their plasma levels over the investigated time course were successfully determined. Copyright \u00a9 2001 John Wiley &amp; Sons, Ltd.<jats:def-list>\n<jats:title>Abbreviations used:<\/jats:title>\n<jats:def-item>\n<jats:term>CX<\/jats:term>\n<jats:def>\n\n<jats:p>cyclohexylamine<\/jats:p>\n<\/jats:def>\n<\/jats:def-item>\n<jats:def-item>\n<jats:term>DIB\u2010Cl<\/jats:term>\n<jats:def>\n\n<jats:p>4\u2010(4,5\u2010diphenyl\u20101<jats:italic>H<\/jats:italic>\u2010imidazole\u20102\u2010yl)benzoyl chloride<\/jats:p>\n<\/jats:def>\n<\/jats:def-item>\n<jats:def-item>\n<jats:term>E<\/jats:term>\n<jats:def>\n\n<jats:p>ephedrine<\/jats:p>\n<\/jats:def>\n<\/jats:def-item>\n<jats:def-item>\n<jats:term>Fen<\/jats:term>\n<jats:def>\n\n<jats:p>fenfluramine<\/jats:p>\n<\/jats:def>\n<\/jats:def-item>\n<jats:def-item>\n<jats:term>FLX<\/jats:term>\n<jats:def>\n\n<jats:p>fluoxetine<\/jats:p>\n<\/jats:def>\n<\/jats:def-item>\n<jats:def-item>\n<jats:term>NE<\/jats:term>\n<jats:def>\n\n<jats:p>norephedrine<\/jats:p>\n<\/jats:def>\n<\/jats:def-item>\n<jats:def-item>\n<jats:term>NorFen<\/jats:term>\n<jats:def>\n\n<jats:p>norfen fluramine<\/jats:p>\n<\/jats:def>\n<\/jats:def-item>\n<jats:def-item>\n<jats:term>2\u2010PEA<\/jats:term>\n<jats:def>\n\n<jats:p>2\u2010phenylethylamine<\/jats:p>\n<\/jats:def>\n<\/jats:def-item>\n<jats:def-item>\n<jats:term>Phen<\/jats:term>\n<jats:def>\n\n<jats:p>phentermine.<\/jats:p>\n<\/jats:def>\n<\/jats:def-item>\n<\/jats:def-list><\/jats:p>","DOI":"10.1002\/bmc.85","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T17:23:24Z","timestamp":1030296204000},"page":"379-388","source":"Crossref","is-referenced-by-count":12,"title":["High performance liquid chromatography with UV detection for the simultaneous determination of sympathomimetic amines using 4\u2010(4,5\u2010diphenyl\u20101<i>H<\/i>\u2010imidazole\u20102\u2010yl)benzoyl chloride as a label"],"prefix":"10.1002","volume":"15","author":[{"given":"Amal","family":"Kaddoumi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai","family":"Kubota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihoko N.","family":"Nakashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakatsu","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichiro","family":"Nakashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,9,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00128-5"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1971.tb08659.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/21.1.59"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0893-133X(95)00113-R"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(91)80584-Y"},{"key":"e_1_2_1_7_1","first-page":"15","volume":"258","author":"Caccia S","year":"1982","journal-title":"Archives of International Pharmacodynamic Therapy"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(96)00528-2"},{"key":"e_1_2_1_9_1","first-page":"224","volume":"308","author":"Clineschmidt B. 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