{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:35:05Z","timestamp":1761510905988,"version":"build-2065373602"},"reference-count":23,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2007,5,21]],"date-time":"2007-05-21T00:00:00Z","timestamp":1179705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>TLC and HPLC methods were developed for indirect chiral separation of penicillamine (3,3\u2010dimethylcysteine) enantiomers after derivatization with Marfey's reagent (FDNP\u2010Ala\u2010NH<jats:sub>2<\/jats:sub>) and two of its structural variants, FDNP\u2010Phe\u2010NH<jats:sub>2<\/jats:sub> and FDNP\u2010Val\u2010NH<jats:sub>2<\/jats:sub>. The binary mobile phase of phenol\u2013water (3:1 v\/v) and solvent combinations of acetonitrile and triethylamine phosphate buffer were found to give the best separation in normal and reversed\u2010phase TLC, respectively. The diastereomers were also resolved on a reversed\u2010phase C18 HPLC column with gradient elution of acetonitrile and 0.01 <jats:sc>m<\/jats:sc> trifluoroacetic acid. The results due to these three reagents were compared. The method was successful for checking the enantiomeric impurity of <jats:sc>l<\/jats:sc>\u2010penicillamine in <jats:sc>d\u2010<\/jats:sc>penicillamine and to check the enantiomeric purity of pharmaceutical formulations of <jats:sc>d\u2010<\/jats:sc>penicillamine. The method was validated for linearity, repeatability, limit of detection and limit of quantification. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.854","type":"journal-article","created":{"date-parts":[[2007,5,26]],"date-time":"2007-05-26T19:47:31Z","timestamp":1180208851000},"page":"1064-1068","source":"Crossref","is-referenced-by-count":35,"title":["Indirect resolution of enantiomers of penicillamine by TLC and HPLC using Marfey's reagent and its variants"],"prefix":"10.1002","volume":"21","author":[{"given":"R.","family":"Bhushan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Br\u00fcckner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virender","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,5,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02312891"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.96"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-004-0118-0"},{"key":"e_1_2_1_5_1","first-page":"126","article-title":"TLC resolution of DL amino acids on impregnated silica gel plates","volume":"7","author":"Bhushan R","year":"1994","journal-title":"Journal of Planar Chromatography\u2014Modern TLC"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-0801(199709)11:5<286::AID-BMC698>3.0.CO;2-9"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(99)00203-4"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200390019"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6468(08)70160-7"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)87169-7"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02269735"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)93517-4"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdd.2510010205"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrc.1270061214"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(92)85409-M"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/a800712h"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92382-C"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1081\/JLC-100100463"},{"key":"e_1_2_1_19_1","first-page":"395","article-title":"Quantitative determination of small amounts of l\u2010penicillamine in d\u2010penicillamine after desulfurization with Raney nickel","volume":"30","author":"Lodemann E","year":"1980","journal-title":"Arzneimittel\u2010Forschung"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02908688"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.19863190515"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01772356"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1130090207"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5173(80)90009-5"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.854","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.854","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:24:05Z","timestamp":1760037845000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.854"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,21]]},"references-count":23,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1002\/bmc.854"],"URL":"https:\/\/doi.org\/10.1002\/bmc.854","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2007,5,21]]}}}