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This method employed high\u2010performance liquid chromatography\u2013tandem mass spectrometry with electrospray ionization. The analytes were monitored in multiple reaction monitoring mode and the polarity was switched from positive to negative to positive to detect all compounds after a single injection. A combination of liquid\u2013liquid extraction and protein precipitation was used to extract all compounds, with at least 75% recovery for all analytes. Within\u2010 and between\u2010day accuracies were at least 85% for 1\u2013500 ng\/mL for nelfinavir, indinavir and abacavir, 10\u2013500 ng\/mL for didanosine and stavudine and 5\u2013500 ng\/mL for all other compounds. This method is very effective for quantifying and screening antiretroviral drugs in clinical samples with limited (50 \u00b5L) volumes. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.865","type":"journal-article","created":{"date-parts":[[2007,6,21]],"date-time":"2007-06-21T07:47:45Z","timestamp":1182412065000},"page":"1095-1104","source":"Crossref","is-referenced-by-count":177,"title":["Simultaneous determination of 17 antiretroviral drugs in human plasma for quantitative analysis with liquid chromatography\u2013tandem mass spectrometry"],"prefix":"10.1002","volume":"21","author":[{"given":"Byung Hwa","family":"Jung","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naser L.","family":"Rezk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arlene S.","family":"Bridges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda H.","family":"Corbett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela D. 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