{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T14:45:51Z","timestamp":1791125151592,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2001,9,4]],"date-time":"2001-09-04T00:00:00Z","timestamp":999561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Identification of glycosaminoglycans (GAGs) synthesized by three human leukaemic cell lines\u2014Jurkat (T\u2010cell leukaemia), Daudi (Burkitt's lymphoma, B\u2010cell leukaemia) and THP\u20101 (acute monocytic leukemia)\u2014and normal peripheral blood mononuclear cells (PBMC) and their distribution among cell membrane and culture medium were studied. GAGs were isolated using ion\u2010exchange chromatography on DEAE\u2010Sephacel and their composition and fine chemical structure were studied using high\u2010performance liquid chromatography with radiochemical detection. All cell lines synthesize chondroitin sulphate (CS) and heparan sulphate (HS) in both cell membrane and culture medium. No hyaluronan was detected using treatment with specific lyases and highly sensitive HPLC methodology. CS is the major secreted GAG in all cell lines tested and the major cell retained GAG in Jurkat and Daudi. HS is the major GAG in the cell membrane of THP\u20101. The amounts of distinct GAGs synthesized by all cancer cell lines differ from those produced by normal PBML indicating a major role of GAGs in malignant transformation of human lymphocytes and monocytes. Copyright \u00a9 2001 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <jats:p>\n                    <jats:def-list>\n                      <jats:title>Abbreviations used:<\/jats:title>\n                      <jats:def-item>\n                        <jats:term>CS<\/jats:term>\n                        <jats:def>\n                          <jats:p>chondroitin sulphate<\/jats:p>\n                        <\/jats:def>\n                      <\/jats:def-item>\n                      <jats:def-item>\n                        <jats:term>GAGs<\/jats:term>\n                        <jats:def>\n                          <jats:p>glycosaminoglycans<\/jats:p>\n                        <\/jats:def>\n                      <\/jats:def-item>\n                      <jats:def-item>\n                        <jats:term>HS<\/jats:term>\n                        <jats:def>\n                          <jats:p>heparan sulphate<\/jats:p>\n                        <\/jats:def>\n                      <\/jats:def-item>\n                      <jats:def-item>\n                        <jats:term>PBMC<\/jats:term>\n                        <jats:def>\n                          <jats:p>peripheral blood mononuclear cells<\/jats:p>\n                        <\/jats:def>\n                      <\/jats:def-item>\n                      <jats:def-item>\n                        <jats:term>PG<\/jats:term>\n                        <jats:def>\n                          <jats:p>proteoglycans PMSF, phenylmethylsulphonyl fluoride<\/jats:p>\n                        <\/jats:def>\n                      <\/jats:def-item>\n                    <\/jats:def-list>\n                  <\/jats:p>","DOI":"10.1002\/bmc.91","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T17:24:42Z","timestamp":1030296282000},"page":"413-417","source":"Crossref","is-referenced-by-count":17,"title":["Synthesis and distribution of glycosaminoglycans in human leukemic B\u2010 and T\u2010cells and monocytes studied using specific enzymic treatments and high\u2010performance liquid chromatography"],"prefix":"10.1002","volume":"15","author":[{"given":"E.","family":"Makatsori","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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