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The supernatant was removed and KTZ enantiomers and amiodarone, the internal standard, were extracted using liquid\u2013liquid extraction with tert\u2010butyl methyl ether. After transfer and evaporation of the organic layer, the residue was reconstituted in mobile phase and injected into the HPLC through a chiral column. The mobile phase consisted of hexane:ethanol:2\u2010propanol with diethyl amine, pumped at 1.5 mL\/min. All components eluted within 18 min. KTZ enantiomers were baseline resolved and peaks were symmetrical in appearance with no interferences. Calibration curves were linear over the range 62.5\u20135000 ng\/mL of enantiomer. The intraday and interday CV% assessments were \u226419 and &lt;13%, respectively, and mean error was &lt;4% for both enantiomers. The lower limit of quantitation was 62.5 ng\/mL for each enantiomer based on 100 \u00b5L rat plasma. In rats, plasma concentrations of (+)\u2010KTZ were higher than those of antipode after single oral doses. The assay was shown to be sensitive and appropriate for use in pharmacokinetics study of KTZ in rat. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.967","type":"journal-article","created":{"date-parts":[[2008,1,18]],"date-time":"2008-01-18T08:47:58Z","timestamp":1200646078000},"page":"542-547","source":"Crossref","is-referenced-by-count":27,"title":["A stereospecific high\u2010performance liquid chromatographic assay for the determination of ketoconazole enantiomers in rat plasma"],"prefix":"10.1002","volume":"22","author":[{"given":"Dalia A.","family":"Hamdy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dion R.","family":"Brocks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,1,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)84319-4"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.530040513"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-022X(00)00060-9"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-022X(02)00119-7"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdd.517"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1875-9114.1995.tb02863.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500100"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199401273300407"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.10294"},{"key":"e_1_2_1_11_1","volume-title":"Fourth International Symposium on Chiral Discrimination","author":"Gal J","year":"1993"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.2903"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600780902"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfm012"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425255.3.2.149"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018996524141"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/S0090-9556(25)06833-3","article-title":"The disposition and pharmacokinetics of ketoconazole in the rat","volume":"15","author":"Remmel RP","year":"1987","journal-title":"Drug Metabolism and Disposition"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)80784-7"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm00093a015"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00570136"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1051\/analusis:1999270713"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.farmac.2003.11.019"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.03.010"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00253-9"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.967","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.967","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:22:33Z","timestamp":1760044953000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.967"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1,18]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2008,5]]}},"alternative-id":["10.1002\/bmc.967"],"URL":"https:\/\/doi.org\/10.1002\/bmc.967","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1,18]]}}}