{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T03:55:19Z","timestamp":1768449319323,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2008,3,18]],"date-time":"2008-03-18T00:00:00Z","timestamp":1205798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The increasing number of samples and target substances in doping control requires continuously improved screening methods, combining high\u2010throughput analysis, simplified sample preparation, robustness and reliability. Hence, a rapid screening procedure based on liquid chromatography\u2013electrospray ionization\u2013tandem mass spectrometry with in\u2010source collision\u2010induced dissociation was developed. The detection of the polysaccharide\u2010based plasma volume expanders dextran and hydroxyethyl starch (HES) in human urine was established without further sample preparation. The in\u2010source fragmentation strategy of the approach represented a valuable tool in the analysis of the polysaccharide\u2010based compounds, allowing the use of tandem mass spectrometry. After direct injection of urine specimens, analytes were chromatographically separated on a monolithic reverse\u2010phase column and detected via multiple reaction monitoring of diagnostic ions at detection limits of 10 \u00b5g\/mL for HES and 30 \u00b5g\/mL for dextran. Validation was performed regarding the parameters specificity, linearity, precision (8\u201318%) and accuracy (77\u2013105%) and the method was applied to the investigation of approximately 400 doping control samples and seven dextran and two hydroxyethyl starch post\u2010administration samples. The approach demonstrated its capability as a rapid screening tool for the detection of dextran and hydroxyethyl starch and represents an alternative to existing screening procedures since time consuming hydrolysis or derivatization steps were omitted. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.986","type":"journal-article","created":{"date-parts":[[2008,3,18]],"date-time":"2008-03-18T06:56:23Z","timestamp":1205823383000},"page":"695-701","source":"Crossref","is-referenced-by-count":21,"title":["Rapid screening of polysaccharide\u2010based plasma volume expanders dextran and hydroxyethyl starch in human urine by liquid chromatography\u2013tandem mass spectrometry"],"prefix":"10.1002","volume":"22","author":[{"given":"Sven","family":"Guddat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Thevis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilhelm","family":"Sch\u00e4nzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,3,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-994204"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1373\/49.7.1041"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(97)00124-4"},{"key":"e_1_2_1_5_1","first-page":"371","volume-title":"Recent Advances in Doping Analysis","author":"Avois L","year":"2004"},{"key":"e_1_2_1_6_1","volume-title":"Cinical Sports Nutrition","author":"Burke L","year":"1994"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.763"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.02.044"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.509"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1491"},{"key":"e_1_2_1_11_1","first-page":"383","volume-title":"Recent Advances in Doping Analysis","author":"Gutierrez\u2010Gallego R","year":"2005"},{"key":"e_1_2_1_12_1","unstructured":"ICH (International Conference on Harmonization).Validation of Analytical Procedures: Text and Methodology Q2(R1);www.ich.org\/LOB\/media\/MEDIA417.pdf(accessed August2007)."},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9888(199807)33:7<644::AID-JMS667>3.0.CO;2-F"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1927"},{"key":"e_1_2_1_15_1","volume-title":"The Statistical Analyses of Experimental Data","author":"Mandel J","year":"1964"},{"key":"e_1_2_1_16_1","first-page":"761","volume-title":"The Extra Pharmacopoeia","author":"Martindale W","year":"1996"},{"key":"e_1_2_1_17_1","first-page":"63","article-title":"Fluid replacement: the American College of Sports Medicine Position Stand","volume":"9","author":"Murray B","year":"1996","journal-title":"Sports Science Exchange"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-994242"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150191105"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0268-960X(05)80021-9"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9888(200001)35:1<77::AID-JMS916>3.0.CO;2-L"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00251-6"},{"key":"e_1_2_1_23_1","unstructured":"World Anti\u2010Doping Agency.The 2007 Prohibited List of Substances 2007;www.wada\u2010ama.org\/docs\/web\/standards_harmonization\/code\/list_standard_2007.pdf(accessed August 2007)."},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(01)00305-1"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.986","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.986","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:35:20Z","timestamp":1760034920000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.986"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,3,18]]},"references-count":23,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2008,7]]}},"alternative-id":["10.1002\/bmc.986"],"URL":"https:\/\/doi.org\/10.1002\/bmc.986","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,3,18]]}}}