{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T11:27:33Z","timestamp":1783078053541,"version":"3.54.6"},"reference-count":52,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T00:00:00Z","timestamp":1540252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gencor Pacific Ltd"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      In Prader\u2013Willi syndrome (PWS), nonprotein coding small nucleolar (sno) RNAs are involved in the paternally deleted region of chromosome 15q11.2\u2010q13, which is believed to cause the hyperphagic phenotype of PWS. Central to this is\n                      <jats:italic>SnoRNA116<\/jats:italic>\n                      . The supplement\n                      <jats:italic>Caralluma fimbriata<\/jats:italic>\n                      extract (CFE) has been shown to decrease appetite behavior in some individuals with PWS. We therefore investigated the mechanism underpinning the effect of CFE on food intake in the\n                      <jats:italic>Snord116del<\/jats:italic>\n                      mouse. Experiments utilized appetite stimulants which included a 5\u2010hydroxytryptamine (5\u2010HT) 2c receptor antagonist (SB242084), as the 5\u2010HT2cR is implicated in central signaling of satiety.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      After 9\u2010week chronic CFE treatment (33\u00a0mg or 100\u00a0mg\u00a0kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      \u00a0day\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) or placebo, the 14\u2010week\u2010old\n                      <jats:italic>Snord116del<\/jats:italic>\n                      (SNO) and wild\u2010type mice (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a072) were rotated through intraperitoneal injections of (a) isotonic saline; (b) 400\u00a0mg\/kg of 2\u2010deoxyglucose (2DG) (glucose deprivation); (c) 100\u00a0mglkg beta\u2010mercaptoacetate (MA), fatty acid signaling; and (d) SB242084 (a selective 5HT2cR antagonist), with 5\u00a0days between reagents. Assessments of food intake were from baseline to 4\u00a0hr, followed by immunohistochemistry of neural activity utilizing c\u2010Fos, neuropeptide Y, and alpha\u2010melanocyte\u2010stimulating hormone within hypothalamic appetite pathways.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Caralluma fimbriata<\/jats:italic>\n                      extract administration decreased food intake more strongly in the SNO100CFE group with significantly stimulated food intake demonstrated during coadministration with SB242084. Though stimulatory deprivation was expected to stimulate food intake, 2DG and MA resulted in lower intake in the\n                      <jats:italic>snord116del<\/jats:italic>\n                      mice compared to the WT animals (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0&lt;0.001). Immunohistochemical mapping of hypothalamic neural activity was consistent with the behavioral studies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study identifies a role for the 5\u2010HT2cR in CFE\u2010induced appetite suppression and significant stimulatory feeding disruptions in the\n                      <jats:italic>snord116del<\/jats:italic>\n                      mouse model.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.1102","type":"journal-article","created":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:26:33Z","timestamp":1540340793000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<i>Caralluma fimbriata<\/i>\n                    extract activity involves the 5\u2010HT2c receptor in PWS\n                    <i>Snord116<\/i>\n                    deletion mouse model"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2585-3758","authenticated-orcid":false,"given":"Joanne L.","family":"Griggs","sequence":"first","affiliation":[{"name":"College of Health and Biomedicine Victoria University Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"Mathai","sequence":"additional","affiliation":[{"name":"College of Health and Biomedicine Victoria University Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puspha","family":"Sinnayah","sequence":"additional","affiliation":[{"name":"College of Health and Biomedicine Victoria University Melbourne Victoria 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