{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:39:29Z","timestamp":1790105969055,"version":"4.0.1"},"reference-count":49,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,7,9]],"date-time":"2019-07-09T00:00:00Z","timestamp":1562630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81373240"],"award-info":[{"award-number":["81373240"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671865"],"award-info":[{"award-number":["81671865"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Chaperone\u2010mediated autophagy (CMA) is an autophagy\u2013lysosome pathway (ALP) that is different from the other two lysosomal pathways, namely, macroautophagy and microautophagy, and can selectively degrade cytosolic proteins in lysosomes without vesicle formation. CMA activity declines in neurodegenerative diseases such as Parkinson's disease, and similar neurotoxicity can occur after methamphetamine (METH) treatment. The relationship between CMA and METH\u2010induced neurotoxicity is not clear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We detected changes in the chaperone protein Hsc70 and the lysosomal surface receptor Lamp\u20102a after METH treatment and then regulated these two proteins by small interfering RNA and DNA plasmid transfection to investigate how CMA influences METH\u2010induced neurotoxicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that CMA activity is decreased after METH exposure in neurons and downregulated Lamp\u20102a can aggravate the neurotoxicity induced by \u03b1\u2010Syn after METH exposure and that Hsc70 overexpression can relieve the abnormal levels of alpha\u2010synuclein and its aggregate forms and the increase in cell apoptosis induced by METH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results provide in vivo evidence for CMA plays a pivotal role in METH\u2010induced neurotoxicity, and upregulation of Hsc70 expression significantly protects neuronal cells against METH\u2010induced toxicity. This research may pave the way for potential therapeutic approaches targeting CMA for METH abuse and neurodegenerative disorders.<\/jats:p><\/jats:sec>","DOI":"10.1002\/brb3.1352","type":"journal-article","created":{"date-parts":[[2019,7,9]],"date-time":"2019-07-09T08:01:15Z","timestamp":1562659275000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["The role of chaperone\u2010mediated autophagy in neurotoxicity induced by alpha\u2010synuclein after methamphetamine exposure"],"prefix":"10.1002","volume":"9","author":[{"given":"Leping","family":"Sun","sequence":"first","affiliation":[{"name":"School of Forensic Medicine Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongling","family":"Lian","sequence":"additional","affiliation":[{"name":"School of Forensic Medicine Southern Medical University Guangzhou 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