{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:33:06Z","timestamp":1759336386693,"version":"3.41.2"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,28]],"date-time":"2020-02-28T00:00:00Z","timestamp":1582848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"publisher","award":["VEGA Grant 2\/0066\/17"],"award-info":[{"award-number":["VEGA Grant 2\/0066\/17"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Synaptic cell adhesion molecule 1 (SynCAM1) also known as cell adhesion molecule 1 (CADM1) is a transmembrane cell adhesion protein that operates in a variety of physiological and pathological cellular contexts, and its interaction with the PDZ signalling protein MUPP1 have been previously implicated in autism spectrum disorder (ASD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used in vitro pull\u2010down systems based on the bacterial and mammalian extracts to study SynCAM1\/CADM1 PDZ interactions with MUPP1 at various conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>So far, the investigated interaction of SynCAM1\/CADM1 with MUPP1 has been mostly attributed to an unspecified region of MUPP1 PDZ domains 1\u20135 or exclusively to domain 2, using a yeast two\u2010hybrid system. We also confirmed the single interaction of native synaptosomal CADM1 with PDZ domain 2. However, in this work, using recombinant proteins overexpressed in bacteria, we found an in vitro pull\u2010down conditions in which all first five domains and, to a much lesser extent, MUPP1 domains 7 and 11 significantly interacted with the whole C\u2010terminal domain of SynCAM1\/CADM1. These PDZ interactions were confirmed by a pull\u2010down assay using the last seven amino acids of the SynCAM1\/CADM1 PDZ motif and using two fusion partners. Multiple interactions were additionally replicated using the continuous N\u2010terminal MUPP1 protein fragment, which included first five PDZ domains, containing either intact or mutated domain 2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We hypothesize that multiple interactions might exist in vivo<jats:italic>,<\/jats:italic> representing transient low\u2010affinity interactions or alternative binding sites on MUPP1 when domain 2 is occupied or occluded by the interaction with other ligands. This newly identified interactions extend the potential genetic mutations, possibly affecting SynCAM1\/CADM1\/MUPP1 function. Possible reasons for the absence of some of the identified CADM1 PDZ interactions in mammalian extracts are discussed.<\/jats:p><\/jats:sec>","DOI":"10.1002\/brb3.1587","type":"journal-article","created":{"date-parts":[[2020,2,28]],"date-time":"2020-02-28T14:59:59Z","timestamp":1582901999000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of SynCAM1\/CADM1 PDZ interactions with MUPP1 using mammalian and bacterial pull\u2010down systems"],"prefix":"10.1002","volume":"10","author":[{"given":"Martina","family":"Baliova","sequence":"first","affiliation":[{"name":"Laboratory of Neurobiology Institute of Molecular Biology Slovak Academy of Sciences Bratislava Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5495-7690","authenticated-orcid":false,"given":"Frantisek","family":"Jursky","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology Institute of Molecular Biology Slovak Academy of Sciences Bratislava 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