{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:02:40Z","timestamp":1791342160080,"version":"4.3.1"},"reference-count":61,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,15]],"date-time":"2020-08-15T00:00:00Z","timestamp":1597449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005367","name":"American Foundation for Surgery of the Hand","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005367","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Posttraumatic scarring of peripheral nerves produces unwanted adhesions that block axonal growth. In the context of surgical nerve repair, the organization of the scar tissue adjacent to conduits used to span the gap between the stumps of transected nerves is poorly understood. The goal of this study was to elucidate the patterns of distribution of collagen\u2010rich scar tissue and analyze the spatial organization of cells that produce fibrotic deposits around and within the conduit's lumen.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Employing a rabbit model of sciatic nerve transection injury, we studied the formation of collagen\u2010rich scar tissue both inside and outside conduits used to bridge the injury sites. Utilizing quantitative immunohistology and Fourier\u2010transform infrared spectroscopy methods, we measured cellular and structural elements present in the extraneural and the intraneural scar of the proximal and distal nerve fragments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Analysis of cells producing collagen\u2010rich deposits revealed that alpha\u2010smooth muscle actin\u2010positive myofibroblasts were only present in the margins of the stumps. In contrast, heat shock protein 47\u2010positive fibroblasts actively producing collagenous proteins were abundant within the entire scar tissue. The most prominent site of transected sciatic nerves with the highest number of cells actively producing collagen\u2010rich scar was the proximal stump.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings suggest the proximal region of the injury site plays a prominent role in pro\u2010fibrotic processes associated with the formation of collagen\u2010rich deposits. Moreover, they show that the role of canonical myofibroblasts in peripheral nerve regeneration is limited to wound contracture and that a distinct population of fibroblastic cells produce the collagenous proteins that form scar tissue. As scarring after nerve injury remains a clinical problem with poor outcomes due to incomplete nerve recovery, further elucidation of the cellular and spatial aspects of neural fibrosis will lead to more targeted treatments in the clinical setting.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.1802","type":"journal-article","created":{"date-parts":[[2020,8,15]],"date-time":"2020-08-15T07:16:06Z","timestamp":1597475766000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Collagen\u2010rich deposit formation in the sciatic nerve after injury and surgical repair: A study of collagen\u2010producing cells in a rabbit model"],"prefix":"10.1002","volume":"10","author":[{"given":"Jolanta","family":"Fertala","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Sidney Kimmel Medical College Thomas Jefferson University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Rivlin","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Sidney Kimmel Medical College Thomas Jefferson University Philadelphia PA USA"},{"name":"Rothman Institute of Orthopaedics Thomas Jefferson University Hospital Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark L.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Sidney Kimmel Medical College Thomas Jefferson University Philadelphia PA USA"},{"name":"Rothman Institute of Orthopaedics Thomas Jefferson University Hospital Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro K.","family":"Beredjiklian","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Sidney Kimmel Medical College Thomas Jefferson University Philadelphia PA USA"},{"name":"Rothman Institute of Orthopaedics Thomas Jefferson University Hospital Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Steplewski","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Sidney Kimmel Medical College Thomas Jefferson University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9153-1912","authenticated-orcid":false,"given":"Andrzej","family":"Fertala","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Sidney Kimmel Medical College Thomas Jefferson University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjps.2004.05.012"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.wnr.0000230519.39456.ea"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2006.05.014"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(79)90188-2"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0282(2001)62:1<1::AID-BIP10>3.0.CO;2-O"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-011-0874-3"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(20000221)417:4<415::AID-CNE3>3.0.CO;2-9"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-014-8862-y"},{"issue":"2","key":"e_1_2_10_10_1","first-page":"593","article-title":"Schwann cell extracellular matrix molecules and their receptors","volume":"15","author":"Chernousov M. 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