{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T15:24:51Z","timestamp":1791473091709,"version":"4.3.4"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T00:00:00Z","timestamp":1598313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Clinical medical research center project of Qinghai Province","award":["2017-SF-L1"],"award-info":[{"award-number":["2017-SF-L1"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Sequelae of stroke were mainly caused by neuronal injury. Oxygen is a key factor affecting the microenvironment of neural stem cells (NSCs), and oxygen levels are used to promote NSC neurogenesis. In this study, effects of intermittent hypoxic preconditioning (HPC) on neurogenesis were investigated in a rat model of middle cerebral artery occlusion (MCAO).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      SD rats were used to establish the MCAO model. Nissl staining and Golgi staining were used to confirm the neuronal injury status in the MCAO model. Immunofluorescence, transmission electron microscopy, Western blot, and qPCR were used to observe the effects of HPC on neurogenesis. At the same time, the hypothesis that HPC could affect proliferation, apoptosis, differentiation, and migration of NSC was verified\n                      <jats:italic>in vitro<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Hypoxic preconditioning significantly ameliorated the neuronal injury induced by MCAO. Compared with MCAO group, the dendrites, Edu\n                      <jats:sup>+<\/jats:sup>\n                      \/SOX2\n                      <jats:sup>+<\/jats:sup>\n                      , Edu\n                      <jats:sup>+<\/jats:sup>\n                      \/DCX\n                      <jats:sup>+<\/jats:sup>\n                      , Edu\n                      <jats:sup>+<\/jats:sup>\n                      \/NeuN\n                      <jats:sup>+<\/jats:sup>\n                      , Edu\n                      <jats:sup>+<\/jats:sup>\n                      \/GFAP\n                      <jats:sup>+<\/jats:sup>\n                      , and Edu\n                      <jats:sup>+<\/jats:sup>\n                      \/Tubulin\n                      <jats:sup>+<\/jats:sup>\n                      positive cells in the HPC\u00a0+\u00a0MCAO group exhibited significantly difference. Similarly, axonal and other neuronal injuries in the HPC\u00a0+\u00a0MCAO group were also ameliorated. In the in vitro experiments, mild HPC significantly enhanced the viability of NSCs, promoted the migration of differentiated cells, and reduced apoptosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our results showed that HPC significantly promotes neurogenesis after MCAO and ameliorates neuronal injury.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.1804","type":"journal-article","created":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T03:00:47Z","timestamp":1598410847000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Hypoxic preconditioning ameliorated neuronal injury after middle cerebral artery occlusion by promoting neurogenesis"],"prefix":"10.1002","volume":"10","author":[{"given":"Lu","family":"Huang","sequence":"first","affiliation":[{"name":"Research Center for High Altitude Medicine Qinghai University  Xining China"},{"name":"Key Laboratory of Application and Foundation for High Altitude Medicine Research in Qinghai Province (Qinghai\u2010Utah Joint Research Key Lab for High Altitude Medicine)  Xining China"},{"name":"Qinghai Provincial People\u2019s Hospital  Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaqi","family":"Wan","sequence":"additional","affiliation":[{"name":"Qinghai Provincial People\u2019s Hospital  Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhancui","family":"Dang","sequence":"additional","affiliation":[{"name":"Qinghai University Medical College  Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Yang","sequence":"additional","affiliation":[{"name":"Qinghai 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