{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T03:26:23Z","timestamp":1772853983660,"version":"3.50.1"},"reference-count":102,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T00:00:00Z","timestamp":1600387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Cognitive deficits during aging are pervasive across species and learning paradigms. One of the major mechanisms thought to play a role in age\u2010related memory decline is dysregulated calcium (Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ) homeostasis. Aging is associated with impaired function of several calcium\u2010regulatory mechanisms, including calcium\u2010binding proteins that normally support intracellular Ca\n                      <jats:sup>2+<\/jats:sup>\n                      regulation. This age\u2010related calcium\u2010binding protein dysfunction and changes in expression lead to disrupted maintenance of intracellular Ca\n                      <jats:sup>2+<\/jats:sup>\n                      , thus contributing to memory decline. Other work has found that age\u2010related cognitive deficits can be mitigated by either blocking Ca\n                      <jats:sup>2+<\/jats:sup>\n                      entry into the cytosol or preventing its release from intracellular Ca\n                      <jats:sup>2+<\/jats:sup>\n                      stores. However, the effect of calcium\u2010binding protein administration on cognitive function during aging is not well\u2010understood. Our laboratory has previously shown that the calcium\u2010binding protein apoaequorin (AQ) is neuroprotective during oxygen\u2013glucose deprivation, a model of in vitro ischemia characterized by calcium\u2010induced excitotoxicity. The current experiments assessed the effect of direct dorsal hippocampal AQ infusion on trace and context fear memory in adult and aged rats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Adult (3\u20136\u00a0months) and aged (22\u201326\u00a0months) male F344 rats were randomly assigned to different experimental infusion groups before undergoing trace fear conditioning and testing. In experiment 1, rats received bilateral dorsal hippocampal infusions of either vehicle or AQ (4% w\/v) 24\u00a0hr before trace fear conditioning. In experiment 2, rats received bilateral dorsal hippocampal infusions of either vehicle or 4% AQ 1\u00a0hr before trace fear conditioning and 1\u00a0hr before testing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Aged rats displayed impaired trace and context fear memory. While a single AQ infusion 24\u00a0hr before trace fear conditioning was insufficient to rescue age\u2010related trace fear memory deficits, AQ infusion 1\u00a0hr before both conditioning and testing abolished age\u2010related context fear memory deficits.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These results suggest that intrahippocampal infusion of AQ may reverse aging\u2010related deficits in hippocampus\u2010dependent context fear memory.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.1832","type":"journal-article","created":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T09:34:54Z","timestamp":1600421694000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Apoaequorin differentially modulates fear memory in adult and aged 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