{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T14:27:16Z","timestamp":1774362436443,"version":"3.50.1"},"reference-count":69,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,2,16]],"date-time":"2021-02-16T00:00:00Z","timestamp":1613433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      PCNSL is a rare extranodal NHL with poor prognosis. Tumorigenesis has been associated with hyperactivation of BCR downstream and NFkB pathways. We studied the prognosis of the relative expression profile of target genes of NFkB pathway (\n                      <jats:italic>MYC, BCL2)<\/jats:italic>\n                      , the essential transcriptional regulator in hematopoiesis\n                      <jats:italic>LMO2,<\/jats:italic>\n                      the checkpoint regulation pathway\n                      <jats:italic>MGMT<\/jats:italic>\n                      , the transcription factor\n                      <jats:italic>POU2F1,<\/jats:italic>\n                      the immune checkpoint gene\n                      <jats:italic>PDCD1,<\/jats:italic>\n                      and the proto\u2010oncogene and transcriptional repressor gene\n                      <jats:italic>BCL6<\/jats:italic>\n                      and its proteins in PCNSL.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study is a retrospective cohort study; 35 immunocompetent PCNSL\u2010DLBCL patients had their gene expression (RT\u2010qPCR) normalized to internal control gene\n                      <jats:italic>GUSB<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Median patient age was 62\u00a0years, median OS was 42.6\u00a0months (95% CI: 26.6\u201358.6), PFS was 41\u00a0months (95% CI: 19.7\u201362.4), and DFS was 59.2\u00a0months (95% CI 31.9\u201386.6). A moderate correlation was found between the gene\/protein expressions of MYC (kappa\u00a0=\u00a00.596,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.022) and of BCL2 (kappa\u00a0=\u00a00.426,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.042). Relative gene expression of\n                      <jats:italic>MYC<\/jats:italic>\n                      \u00a0\u2265\u00a00.201 (HR 6.117;\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.003) was associated with worse 5\u2010year OS. Relative gene expression of\n                      <jats:italic>MYC<\/jats:italic>\n                      \u00a0\u2265\u00a00.201 (HR 3.96;\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.016) and\n                      <jats:italic>MGMT<\/jats:italic>\n                      \u00a0\u2265\u00a00.335 (HR 3.749;\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a0.056) was associated with worse PFS. Age\u00a0&gt;\u00a060\u00a0years and IELSG score moderate\/high were also associated with worse prognosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Overexpression of\n                      <jats:italic>MYC<\/jats:italic>\n                      and overexpression of\n                      <jats:italic>MGMT<\/jats:italic>\n                      were prognostic markers associated with unfavorable clinical outcomes in PCNSL.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.2061","type":"journal-article","created":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T13:44:30Z","timestamp":1613742270000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["New genetic prognostic biomarkers in primary central nervous system lymphoma (PCNSL)"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2362-3978","authenticated-orcid":false,"given":"Diego","family":"Gomes Candido Reis","sequence":"first","affiliation":[{"name":"Department of Hematology, Hemotherapy and Cell Therapy Laboratory of Medical Investigation in Pathogenesis and Directed Therapy in Onco\u2010Immuno\u2010Hematology (LIM\u201031) Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP) S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e9bora","family":"Levy","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology (LIM19) Heart Institute (InCor) Faculdade de Medicina Hospital das Cl\u00ednicas HCFMUSP Universidade de S\u00e3o Paulo S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u00eds Alberto de P\u00e1dua Covas","family":"Lage","sequence":"additional","affiliation":[{"name":"Department of 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