{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T21:53:12Z","timestamp":1790545992551,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T00:00:00Z","timestamp":1621814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671298"],"award-info":[{"award-number":["81671298"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81601174"],"award-info":[{"award-number":["81601174"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Accumulating evidence demonstrates that certain microRNAs play critical roles in epileptogenesis. Our previous studies found microRNA (miR)\u2010129\u20132\u20103p was induced in patients with refractory temporal lobe epilepsy (TLE). In this study, we aimed to explore the role of miR\u2010129\u20132\u20103p in TLE pathogenesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      By bioinformatics, we predicted miR\u2010129\u20132\u20103p may target the gene\n                      <jats:italic>GABRA1<\/jats:italic>\n                      encoding the GABA type A receptor subunit alpha 1. Luciferase assay was used to investigate the regulation of miR\u2010129\u20132\u20103p on\n                      <jats:italic>GABRA1<\/jats:italic>\n                      3\u2019UTR. The dynamic expression of miR\u2010129\u20132\u20103p and\n                      <jats:italic>GABRA1<\/jats:italic>\n                      mRNA and protein levels were measured in primary hippocampal neurons and a rat kainic acid (KA)\u2010induced seizure model by quantitative reverse transcription\u2010polymerase chain reaction (qPCR), Western blotting, and immunostaining. MiR\u2010129\u20132\u20103p agomir and antagomir were utilized to explore their role in determining\n                      <jats:italic>GABRA1<\/jats:italic>\n                      expression. The effects of targeting miR\u2010129\u20132\u20103p and\n                      <jats:italic>GABRA1<\/jats:italic>\n                      on epilepsy were assessed by electroencephalography (EEG) and immunostaining.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Luciferase assay, qPCR, and Western blot results suggested\n                      <jats:italic>GABRA1<\/jats:italic>\n                      as a direct target of miR\u2010129\u20132\u20103p. MiR\u2010129\u20132\u20103p level was significantly upregulated, whereas\n                      <jats:italic>GABRA1<\/jats:italic>\n                      expression downregulated in KA\u2010treated rat primary hippocampal neurons and KA\u2010induced seizure model. In vivo knockdown of miR\u2010129\u20132\u20103p by antagomir alleviated the seizure\u2010like EEG findings in accordance with the upregulation of\n                      <jats:italic>GABRA1<\/jats:italic>\n                      . Furthermore, the seizure\u2010suppressing effect of the antagomir was partly\n                      <jats:italic>GABRA1<\/jats:italic>\n                      dependent.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The results suggested\n                      <jats:italic>GABRA1<\/jats:italic>\n                      as a target of miR\u2010129\u20132\u20103p in rat primary hippocampal neurons and a rat kainic acid (KA) seizure model. Silencing of miR\u2010129\u20132\u20103p exerted a seizure\u2010suppressing effect in rats. MiR\u2010129\u20132\u20103p\/\n                      <jats:italic>GABRA1<\/jats:italic>\n                      pathway may represent a potential target for the prevention and treatment of refractory epilepsy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.2195","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T10:25:35Z","timestamp":1621851935000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Inhibition of microRNA\u2010129\u20132\u20103p protects against refractory temporal lobe epilepsy by regulating\n                    <i>GABRA1<\/i>"],"prefix":"10.1002","volume":"11","author":[{"given":"Guan\u2010Yu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery the Second Affiliated Hospital of Dalian Medical University  Dalian China"},{"name":"Epileptic Center of Liaoning the Second Affiliated Hospital of 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