{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:17:32Z","timestamp":1790104652199,"version":"4.0.1"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,7,31]],"date-time":"2021-07-31T00:00:00Z","timestamp":1627689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>MicroRNA\u2010410 (miR\u2010410) has been found to be deregulated in neonatal hypoxic\u2010ischemic encephalopathy (HIE). However, the clinical significance and biological function of miR\u2010410 remain largely elusive. This study aims to investigate the expression and diagnostic performance of miR\u2010410 in HIE newborns, and explores the neuroprotective effect of miR\u2010410 in an oxygen\u2010glucose deprivation (OGD)\u2010induced cell injury model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Expression of miR\u2010410 was examined using quantitative real\u2010time PCR, and its diagnostic performance was evaluated using a receiver operating characteristic analysis. We used OGD\u2010injured PC12 and SH\u2010SY5Y cells to construct an\n                      <jats:italic>in vitro<\/jats:italic>\n                      HIE model. The effect of miR\u2010410 on OGD\u2010induced cell injury was analyzed by assessing cell viability and apoptosis. Enzyme\u2010linked immunosorbent assay was used to evaluate inflammation in cell model. A target gene was assessed according to the luciferase reporter assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Serum miR\u2010410 expression was significantly decreased in HIE newborns and OGD\u2010injured cell model. The reduced miR\u2010410 expression served as a biomarker for the diagnosis and progression of HIE. The OGD\u2010induced impaired cell viability, enhanced cell apoptosis, and activated neuroinflammation were abrogated by the overexpression of miR\u2010140 in both PC12 and SH\u2010SY5S cells. Regarding the mechanisms underlying the function of miR\u2010410, phosphatase and tensin homolog (PTEN) was proposed as a direct target of miR\u2010410.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>All data revealed that serum downregulated miR\u2010410 in HIE serves as candidate diagnostic biomarker, and that miR\u2010410 exerts a neuroprotective role in OGD\u2010injured cells by improving cell viability and inhibiting cell apoptosis through targeting PTEN.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.2293","type":"journal-article","created":{"date-parts":[[2021,7,31]],"date-time":"2021-07-31T03:43:59Z","timestamp":1627703039000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["MicroRNA\u2010410 serves as a candidate biomarker in hypoxic\u2010ischemic encephalopathy newborns and provides neuroprotection in oxygen\u2010glucose deprivation\u2010injured PC12 and SH\u2010SY5Y cells"],"prefix":"10.1002","volume":"11","author":[{"given":"Qinghong","family":"Meng","sequence":"first","affiliation":[{"name":"Department of Neonatology Weifang People's Hospital  Weifang Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1874-9151","authenticated-orcid":false,"given":"Peipei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Neonatology Weifang People's Hospital  Weifang Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Weifang People's Hospital  Weifang Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,31]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2016.04.010"},{"key":"e_1_2_8_3_1","first-page":"57","article-title":"Hypoxic\u2010ischemic encephalopathy and other neonatal encephalopathies","volume":"24","author":"Glass H. 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Chinese Journal of Pediatrics"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2018.0409"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.001858"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201700673r"},{"issue":"3","key":"e_1_2_8_22_1","first-page":"1408","article-title":"MiR\u2010155\u20105p accelerates cerebral ischemia\u2010reperfusion injury via targeting DUSP14 by regulating NF\u2010kappaB and MAPKs signaling pathways","volume":"24","author":"Shi Y.","year":"2020","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.01.029"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-019-0257-6"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2014.06.005"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/brb3.835"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-019-0257-6"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2013.02.046"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S197325"}],"container-title":["Brain and Behavior"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/brb3.2293","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/brb3.2293","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/brb3.2293","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T23:45:50Z","timestamp":1692315950000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/brb3.2293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,31]]},"references-count":28,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1002\/brb3.2293"],"URL":"https:\/\/doi.org\/10.1002\/brb3.2293","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/BRB3.2293\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.2293\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.2293\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.2293\/v1\/review1","asserted-by":"object"}]},"ISSN":["2162-3279","2162-3279"],"issn-type":[{"value":"2162-3279","type":"print"},{"value":"2162-3279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,31]]},"assertion":[{"value":"2021-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2293"}}