{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:38:19Z","timestamp":1790703499511,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T00:00:00Z","timestamp":1639353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To evaluate and identify the effects and explore the mechanisms of pyruvate kinase M2 (PKM2) on stroke\u2010induced post stroke depression (PSD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Rats were separated into six different groups, including sham + saline, Stroke + saline, PSD + saline, PSD + recombinant pyruvate kinase M2 (rPKM2) (112\u00a0ng\/kg), PSD + rPKM2 (224\u00a0ng\/kg), and PSD + rPKM2 (224\u00a0ng\/kg) + bevacizumab. Then, the body weight, sucrose preference rate, immobility time, horizontal movement, and vertical movement were determined to evaluate the effect of PKM2 on improving the depressive behavior of PSD rats. Subsequently, the proliferation of oligodendrocytes in subventricular zone (SVZ) of rats in each group was examined by western blot and immunofluorescent staining. Furthermore, the mRNA and protein expression levels of TNF\u2010\u03b1, IL\u20106, and IL\u20101\u03b2 were also detected by qPCR and ELISA to verify the anti\u2010inflammatory effects of PKM2 on PSD rats. In addition, the protein expression levels of MDA, LDH, and NO were tested to reveal that PKM2 can reduce oxidative stress in PSD rats. The western blot and IHC assays were employed to examine the protein expression levels of VEGF, PKM2, and ERK in PSD rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In this study, the results showed that PKM2 can improve the depressive behavior and proliferation of oligodendrocytes in PSD rats. In addition, PKM2 has anti\u2010inflammatory and anti\u2010oxidative stress effects on PSD rats. Meanwhile, PKM2 activated the expression level of VEGF\/MAPK\/ERK pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PKM2 improves symptoms of post\u2010ischemic stroke depression by activating VEGF\u2010mediated MAPK\/ERK pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/brb3.2450","type":"journal-article","created":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T10:12:19Z","timestamp":1639390339000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Pyruvate kinase M2 (PKM2) improve symptoms of post\u2010ischemic stroke depression by activating VEGF to mediate the MAPK\/ERK pathway"],"prefix":"10.1002","volume":"12","author":[{"given":"Yun","family":"Feng","sequence":"first","affiliation":[{"name":"Department of Neurology The Affiliated Hospital of Youjiang Medical University for Nationalities  Baise City Guangxi Province 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