{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T05:20:33Z","timestamp":1776835233829,"version":"3.51.2"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T00:00:00Z","timestamp":1640131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>LncRNA growth arrest\u2010specific transcript 5 (GAS5) has been proven to be involved in autoimmune diseases. Rheumatoid arthritis is a type of autoimmune disease that may affect myasthenia gravis (MG) patients. However, its direct role in MG is unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Our study included 62 generalized MG patients. GAS5 expression was analyzed with real\u2010time quantitative RT\u2010PCR (qRT\u2010PCR). The interaction between GAS5 and interleukin 10 (IL\u201010) was explored in overexpressed cells using real time quantitative polymerase chain reactions (RT\u2010qPCRs) and western blot. The correlation of GAS5 and IL\u201010 was analyzed using Pearson's correlation analysis. The diagnostic value of GAS5 for MG was analyzed using receiver operating characteristic (ROC) curve analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>GAS5 and IL\u201010 mRNA levels in peripheral blood mononuclear cells (PBMCs) were significantly lower in MG patients than healthy controls. Downregulated GAS5 effectively distinguished MG patients from healthy controls. GAS5 expression was positively correlated with IL\u201010 expression in both MG patients and healthy controls. GAS5 overexpression significantly upregulated IL\u201010 expression in PBMCs derived from both MG patients and healthy controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>LncRNA GAS5 may improve generalized MG by positively regulating IL\u201010 expression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.2457","type":"journal-article","created":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T10:26:55Z","timestamp":1640168815000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["LncRNA GAS5 positively regulates IL\u201010 expression in patients with generalized myasthenia gravis"],"prefix":"10.1002","volume":"12","author":[{"given":"Shuangshuang","family":"Peng","sequence":"first","affiliation":[{"name":"Department of Neurology Taizhou First People's Hospital  Taizhou City P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7227-1008","authenticated-orcid":false,"given":"Yangping","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurology Taizhou First People's Hospital  Taizhou City P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2013.12.001"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2013.12.011"},{"issue":"10","key":"e_1_2_9_4_1","first-page":"6776","article-title":"Decreased expression of lncRNA GAS5 predicts a poor prognosis in cervical cancer","volume":"7","author":"Cao S.","year":"2014","journal-title":"International Journal of Clinical\u00a0and Experimental Pathology"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2015.03.018"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3074"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(15)00145-3"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aal3535"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.293.15.1906"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep10159"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12920-015-0087-z"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1254.054"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-1331.2010.03017.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000279334"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2015.1030614"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/acn3.24"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2015.10.023"},{"key":"e_1_2_9_18_1","doi-asserted-by":"crossref","DOI":"10.4049\/jimmunol.196.Supp.124.43","article-title":"IL\u201010 producing regulatory B cells are decreased and functional impaired in myasthenia gravis","volume":"196","author":"Sheng J. R.","year":"2016","journal-title":"Journal of Immunology"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.23938"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/08916934.2020.1846183"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000000835"},{"issue":"7","key":"e_1_2_9_22_1","first-page":"8465","article-title":"Clinical significance and mechanism of LncRNA GAS\u20105 in osteoarthritis","volume":"13","author":"Zhang H.","year":"2021","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-015-0358-1"}],"container-title":["Brain and Behavior"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/brb3.2457","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/brb3.2457","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/brb3.2457","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,26]],"date-time":"2023-08-26T23:51:10Z","timestamp":1693093870000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/brb3.2457"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,22]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1002\/brb3.2457"],"URL":"https:\/\/doi.org\/10.1002\/brb3.2457","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/BRB3.2457\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.2457\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.2457\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.2457\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.2457\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/BRB3.2457\/v3\/response1","asserted-by":"object"}]},"ISSN":["2162-3279","2162-3279"],"issn-type":[{"value":"2162-3279","type":"print"},{"value":"2162-3279","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,22]]},"assertion":[{"value":"2021-07-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2457"}}