{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T04:41:26Z","timestamp":1782362486976,"version":"3.54.5"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,3,20]],"date-time":"2022-03-20T00:00:00Z","timestamp":1647734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To review the clinical symptoms, auxiliary examination findings, and outcomes of patients with nitrous oxide (N<jats:sub>2<\/jats:sub>O) abuse, and analyze the factors that affect outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients with N<jats:sub>2<\/jats:sub>O abuse treated in the Department of Neurology between January 2018 and December 2020 were included. The clinical data of these patients were collected, and follow\u2010up was conducted to determine the outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The average age of the 110 patients with N<jats:sub>2<\/jats:sub>O abuse was 21.4\u00a0\u00b1\u00a04.2 years (range: 14\u201333 years). Clinical presentation primarily included neurological symptoms, such as limb numbness and\/or weakness (97%), psychiatric symptoms, changes in appetite, and skin hyperpigmentation. Laboratory test results were characterized by vitamin B<jats:sub>12<\/jats:sub>deficiency (60%, 34 out of 57 cases) and high homocysteine level (69%, 31 out of 45 cases). Electromyography indicated mixed axonal and demyelination injury (92%, 80 out of 87 cases). Motor and sensory nerves were simultaneously involved, and injury primarily involved the lower limbs. One hundred and seven (97%) patients were clinically diagnosed with peripheral neuropathy, of whom 26 (24%) exhibited spinal abnormalities on magnetic resonance imaging, supporting a diagnosis of subacute combined degeneration. Treatment included N<jats:sub>2<\/jats:sub>O withdrawal and vitamin B<jats:sub>12<\/jats:sub>supplementation. Reexamination of six patients indicated that treatment was effective. Follow\u2010up was completed for 51 patients. Thirty\u2010four patients (67%) recovered completely, 17 patients (33%) had residual limb numbness, and only one patient experienced relapse. Sex was an independent prognostic factor; the outcomes of female patients were better than that of male patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The recreational use of N<jats:sub>2<\/jats:sub>O has largely expanded among youth in recent decades, which has become a growing public health concern in China. It highlights the importance of the recognition of various clinical symptoms, particularly limb numbness and\/or weakness related to the cases of N<jats:sub>2<\/jats:sub>O abuse. The therapeutic administration of vitamin B<jats:sub>12<\/jats:sub>supplementation and N<jats:sub>2<\/jats:sub>O withdrawal can make the overall prognosis good, especially for female patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/brb3.2533","type":"journal-article","created":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T05:18:33Z","timestamp":1647839913000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Analysis of clinical characteristics and prognostic factors in 110 patients with nitrous oxide abuse"],"prefix":"10.1002","volume":"12","author":[{"given":"Miao","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Neurology Shengjing Hospital of China Medical University Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Qiao","sequence":"additional","affiliation":[{"name":"Department of Neurology Shengjing Hospital of China Medical University Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weishuai","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology Shengjing Hospital of China Medical University Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuying","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Neurofunction Shengjing Hospital of China Medical University Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Neurology Shengjing Hospital of China Medical University Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongming","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Neurology Shengjing Hospital of China Medical University Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Neurology Shengjing Hospital of China Medical University Shenyang Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,3,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/j19680002886"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.2344\/0003\u20103006\u201055.4.124"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1983.04050070046009"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0010\u2010440X(75)90065\u20106"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542\u2010194911000\u201000005"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.33.10.909"},{"key":"e_1_2_11_8_1","doi-asserted-by":"crossref","first-page":"bcr2012008509","DOI":"10.1136\/bcr-2012-008509","article-title":"Subacute combined degeneration of the spinal cord following recreational nitrous oxide use","author":"Cheng H. 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