{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:42:15Z","timestamp":1790707335991,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T00:00:00Z","timestamp":1653350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Trigeminal neuralgia (TN) is a neuropathic pain that occurs in branches of the trigeminal nerve. MicroRNAs (miRNAs) have been considered key mediators of neuropathic pain. This study was aimed to elucidate the pathophysiological function and mechanisms of miR\u2010223\u20103p in mouse models of TN.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Infraorbital nerve chronic constriction injury (CCI\u2010ION) was applied in male C57BL\/6J mice to establish mouse models of TN. Pain responses were assessed utilizing Von Frey method. The expression of miR\u2010223\u20103p, MKNK2, and MAPK\/ERK pathway protein in trigeminal ganglions (TGs) of CCI\u2010ION mice was measured using RT\u2010qPCR and Western blotting. The concentrations of inflammatory cytokines were evaluated using Western blotting. The relationship between miR\u2010223\u20103p and MKNK2 was tested by a luciferase reporter assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that miR\u2010223\u20103p was downregulated, while MKNK2 was upregulated in TGs of CCI\u2010ION mice. MiR\u2010223\u20103p overexpression by an intracerebroventricular injection of Lv\u2010miR\u2010223\u20103p attenuated trigeminal neuropathic pain in CCI\u2010ION mice, as well as reduced the protein levels of pro\u2010inflammatory cytokines in TGs of CCI\u2010ION mice. MKNK2 was verified to be targeted by miR\u2010223\u20103p. Additionally, miR\u2010223\u20103p overexpression decreased the phosphorylation levels of ERK1\/2, JNK, and p38 protein in TGs of CCI\u2010ION mice to inhibit MAPK\/ERK signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Overall, miR\u2010223\u20103p attenuates the development of TN by targeting MKNK2 to suppress MAPK\/ERK signaling.<\/jats:p><\/jats:sec>","DOI":"10.1002\/brb3.2634","type":"journal-article","created":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T10:40:03Z","timestamp":1653388803000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["MiR\u2010223\u20103p alleviates trigeminal neuropathic pain in the male mouse by targeting MKNK2 and MAPK\/ERK signaling"],"prefix":"10.1002","volume":"12","author":[{"given":"Bixia","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Neurology The Affiliated Hospital of Putian University  Putian 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