{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T12:28:00Z","timestamp":1789648080263,"version":"4.0.0"},"reference-count":89,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T00:00:00Z","timestamp":1657756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Alzheimer's disease (AD), a common form of dementia, has been reported to influence 27 million individuals globally. Several risk factors including oxidative stress, gut microbiota imbalance, and cognitive activity are reported to be closely associated with the initiation or progression of AD. Although miR\u2010483\u20103p was identified to be downregulated in AD patient serum. However, the biological role and mechanism of miR\u2010483\u20103p remained unknown in AD. Here, we explored the role of miR\u2010483\u20103p in AD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Sprague\u2013Dawley rats were injected with homocysteine (Hcy) to establish an AD animal model. The Morris water maze tests and contextual fear tests were conducted to assess the cognitive and memory abilities of rats. TUNEL staining was utilized to determine cell apoptosis. Luciferase reporter assay was used to evaluate the binding relation between miR\u2010483\u20103p and exportin 1 (XPO1).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Homocysteine treatment (400 \u03bcg\/kg) induced the learning, cognitive and memory defects of rats. miR\u2010483\u20103p was downregulated in Hcy\u2010treated rat hippocampus. Functionally, miR\u2010483\u20103p alleviated cell apoptosis and impairments of learning and memory abilities in Hcy\u2010treated rats. In addition, miR\u2010483\u20103p inhibited cell apoptosis and protein level of AD\u2010associated factors (APP, BACE1, and A\u03b21\u201042) in PC12 cells. In mechanism, miR\u2010483\u20103p was confirmed to target XPO1 in PC12 cells. XPO1 displayed high level in rat hippocampus and was negatively correlated with miR\u2010483\u20103p levels. Finally, XPO1 overexpression rescued the suppressive effect of miR\u2010483\u20103p on cell apoptosis and protein levels of AD\u2010associated factors.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>miR\u2010483\u20103p alleviates neural cell apoptosis and impairments of learning and memory abilities by targeting XPO1 in AD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.2680","type":"journal-article","created":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T03:13:36Z","timestamp":1657768416000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["MiR\u2010483\u20103p improves learning and memory abilities via XPO1 in Alzheimer's disease"],"prefix":"10.1002","volume":"12","author":[{"given":"Gang","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Rehabilitation Gezhouba Central Hospital of Sinopharm, The Third Clinical Medical College of China Three Gorges University  Yichang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2872-8402","authenticated-orcid":false,"given":"Changya","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neurology Hubei Provincial Hospital of Traditional Chinese Medicine  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","unstructured":"Alzheimer's disease facts and figures. 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