{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:01:55Z","timestamp":1772118115237,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T00:00:00Z","timestamp":1666137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012498","name":"Alzheimer Society Research Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012498","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Endothelial nitric oxide synthase (eNOS) produces nitric oxide, which is essential for a variety of physiological functions in the brain. Previous work has demonstrated the detrimental effects of eNOS deficiency on brain function in male eNOS knockout (eNOS KO) mice. However, the effect of eNOS deficiency on brain structure and any association between these effects and sex is unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study used three\u2010dimensional high\u2010resolution ex vivo magnetic resonance imaging and behavioral tests of anxiety and cognitive performance to investigate structure\u2013function relationships in the brain of female and male eNOS KO mice in young adulthood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>While there were no differences in anxiety\u2010like behavior or locomotion, there was a sex\u2010specific deficit in contextual fear memory retention in male, but not in female, eNOS mice compared to wild\u2010type controls. Moreover, we found that eNOS deficiency induced changes in multiple brain regions that are involved in learning and fear memory including the hippocampus, amygdala, hypothalamus, and areas of the cortex. Several of these MRI\u2010detectable neuroanatomical changes were dependent on sex.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The observation that eNOS deficiency impacts brain structure at an early age demonstrates the importance of eNOS for healthy brain development.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.2801","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T09:49:37Z","timestamp":1666172977000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Structural brain abnormalities in endothelial nitric oxide synthase\u2010deficient mice revealed by high\u2010resolution magnetic resonance imaging"],"prefix":"10.1002","volume":"12","author":[{"given":"Hannah","family":"George","sequence":"first","affiliation":[{"name":"Department of Chemistry Memorial University of Newfoundland  St. John's Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grace V.","family":"Mercer","sequence":"additional","affiliation":[{"name":"Department of Chemistry Memorial University of Newfoundland  St. John's Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darcie","family":"Stapleton","sequence":"additional","affiliation":[{"name":"Department of Chemistry Memorial University of Newfoundland  St. John's Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Dawson","sequence":"additional","affiliation":[{"name":"Department of Psychology Memorial University of Newfoundland  St. John's Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phillip E.","family":"MacCallum","sequence":"additional","affiliation":[{"name":"Department of Psychology Memorial University of Newfoundland  St. John's Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoshana","family":"Spring","sequence":"additional","affiliation":[{"name":"Mouse Imaging Centre Hospital for Sick Children  Toronto Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John G.","family":"Sled","sequence":"additional","affiliation":[{"name":"Mouse Imaging Centre Hospital for Sick Children  Toronto Canada"},{"name":"Translational Medicine Hospital for Sick Children  Toronto Canada"},{"name":"Department of Medical Biophysics University of Toronto  Toronto Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline","family":"Blundell","sequence":"additional","affiliation":[{"name":"Department of Psychology Memorial University of Newfoundland  St. John's Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1667-8564","authenticated-orcid":false,"given":"Lindsay S.","family":"Cahill","sequence":"additional","affiliation":[{"name":"Department of Chemistry Memorial University of Newfoundland  St. John's Canada"},{"name":"Discipline of Radiology Memorial University of Newfoundland  St. John's Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,10,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2006.03.005"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2004.08.029"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.12334"},{"key":"e_1_2_8_5_1","first-page":"1","article-title":"Advanced normalization tools (ANTS)","volume":"2","author":"Avants B. 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