{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:14:21Z","timestamp":1781100861850,"version":"3.54.1"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T00:00:00Z","timestamp":1668988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study sought to evaluate the feasibility of multifunctional gastrodin (GAS)\u2010containing nano\u2010drug carrier system against cerebral ischemia\u2013reperfusion injury (CIRI).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The drug\u2010loaded nanocomposite (Au\u2010G5.NHAc\u2010PS\/GAS) with certain encapsulation efficiency (EE) was prepared by physical adsorption method using different proportions of GAS and drug\u2010carrying system (Au\u2010G5.NHAc\u2010PS). High\u2010performance liquid chromatography was used to determine the drug loading and EE. Cultured rat astrocytes and hypothalamic neurons were assigned into four groups: PBS, Au\u2010G5.NHAc\u2010PS, Au\u2010G5.NHAc\u2010PS\/GAS, and GAS. CCK\u20108 assay, flow cytometry, and quantitative real\u2010time PCR were performed to examine the cell viability, apoptosis, and the expression of tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), IL\u20101\u03b2, and IL\u20106 in the astrocytes and hypothalamic neurons, respectively. Cellular uptake of GAS and Au\u2010G5.NHAc\u2010PS\/GAS was analyzed by using Hoechst 33342 staining. The animal model with focal cerebral ischemia was generated by middle cerebral artery occlusion (MCAO) in healthy male Sprague Dawley (SD) rats, and pathological changes of brain tissue and major organs in the rats were identified by hematoxylin and eosin (HE) staining. Apoptosis in rat astrocytes and hypothalamic neurons was detected by TUNEL staining and flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Au\u2010G5.NHAc\u2010PS had a spherical shape with a uniform size of 157.3\u00a0nm. Among the nanoparticles, Au\u2010G5.NHAc\u2010PS\/GAS with an EE of 70.3% displayed the best release delay effect. Moreover, we observed that in vitro cytotoxicity and cellular uptake of Au\u2010G5.NHAc\u2010PS\/GAS were higher than those of GAS, whereas the expression of TNF\u2010\u03b1, IL\u20101\u03b2, and IL\u20106 was significantly downregulated in Au\u2010G5.NHAc\u2010PS\/GAS group as compared to G5.NHAc\u2010PS group. Notably, HE staining revealed that although Au\u2010G5.NHAc\u2010PS\/GAS had no toxic and side effects on the main organs of rats, it alleviated the damage of brain tissue in the MCAO rats. Besides, Au\u2010G5.NHAc\/GAS markedly reduced MCAO\u2010induced apoptosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Au\u2010G5.NHAc\u2010PS showed favorable surface morphology, sustained drug release ability, no measurable toxicity, and good biocompatibility, indicating that GAS exerts anti\u2010inflammatory and antiapoptotic effects on CIRI.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.2810","type":"journal-article","created":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T06:31:35Z","timestamp":1669012295000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Preparation of gastrodin\u2010modified dendrimer\u2010entrapped gold nanoparticles as a drug delivery system for cerebral ischemia\u2013reperfusion injury"],"prefix":"10.1002","volume":"12","author":[{"given":"Wenqiang","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery First Affiliated Hospital of Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanlin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery First Affiliated Hospital of Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanghong","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery First Affiliated Hospital of Kunming Medical University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangqian","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery First Affiliated Hospital of Kunming Medical University  Kunming 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