{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:29:00Z","timestamp":1787164140044,"version":"build-2736575974"},"reference-count":58,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T00:00:00Z","timestamp":1688428800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005071","name":"Shiraz University","doi-asserted-by":"publisher","award":["95INT1M1755"],"award-info":[{"award-number":["95INT1M1755"]}],"id":[{"id":"10.13039\/501100005071","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Multiple sclerosis (MS) is the most common demyelinating disease of the central nervous system. However, the limitations of available therapeutic strategies are frustrating, both in terms of their low efficacy and multiple side effects. Previous studies showed that natural compounds such as Chalcones possess neuroprotective effects on neurodegenerative disorders. However, few studies have so far been published on the potential effects of Chalcones on treating demyelinating disease. The present study was designed to investigate the effects of Chalcones from Ashitaba (ChA) on cuprizone\u2010induced noxious changes in the C57BL6 mice model of MS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The mice received normal diets (Control group: CNT), or Cuprizone\u2010supplemented diets either without ChA (Cuprizone group: CPZ) or with low or high (300, 600\u00a0mg\/kg\/day) doses of ChA (ChA\u2010treated groups: CPZ\n                      <jats:bold>+<\/jats:bold>\n                      ChA300\/600). Brain\u2010derived neurotrophic factor (BDNF) and tumor necrosis factor alpha (TNF\u03b1) levels, demyelination scores in the corpus callosum (CC), and cognitive impairment were evaluated using the enzyme\u2010linked immunosorbent assay, histological, and Y\u2010maze tests, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The findings showed that ChA Co\u2010treatment significantly reduced the extent of demyelination in the CC and the serum and brain levels of TNF\u03b1 in the ChA\u2010treated groups compared to the CPZ group. Besides, treatment with a higher dose of ChA significantly improved the behavioral responses and BDNF levels in the serum and brain of the CPZ\n                      <jats:bold>+<\/jats:bold>\n                      ChA600 group when compared with the CPZ group.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The present study provided evidence for the neuroprotective effects of ChA on cuprizone\u2010induced demyelination and behavioral dysfunction in C57BL\/6 mice, possibly by modulating TNF\u03b1 secretion and BDNF expression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.3144","type":"journal-article","created":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T00:40:58Z","timestamp":1688517658000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The neuroprotective effects of Chalcones from Ashitaba on cuprizone\u2010induced demyelination via modulation of brain\u2010derived neurotrophic factor and tumor necrosis factor \u03b1"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8139-6144","authenticated-orcid":false,"given":"Soodeh","family":"Rowhanirad","sequence":"first","affiliation":[{"name":"Division of Physiology, Department of Basic Science, School of Veterinary Medicine Shiraz University Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8819-1619","authenticated-orcid":false,"given":"Mahnaz","family":"Taherianfard","sequence":"additional","affiliation":[{"name":"Division of Physiology, Department of Basic Science, School of Veterinary Medicine Shiraz University Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2020.173695"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010020\u201000114\u2010z"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2013.08.014"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0042-110496"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20103083.2005.01649.x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/138955711795305335"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25122907"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1401459"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(08)61620\u20107"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2018.05.035"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.12212"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4267\u201013.2014"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.109.057448"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9040945"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1042\/AN20090032"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/350230a0"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/brb3.1388"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401\u2010009\u20100591\u20103"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571\u2010017\u20100510\u20104"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules181214670"},{"key":"e_1_2_11_22_1","first-page":"4761","article-title":"Effects of chalcone derivatives on players of the immune system","volume":"9","author":"Lee J. 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