{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T01:11:34Z","timestamp":1793236294548,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T00:00:00Z","timestamp":1691366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82160934;81960895"],"award-info":[{"award-number":["82160934;81960895"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Central sensitization is one of the important mechanisms underlying neuropathic and radicular pain due to cervical spondylotic radiculopathy (CSR). Recent studies have shown that the calmodulin\u2010dependent protein kinase II (CaMKII)\/cAMP\u2010response element binding protein (CREB)\/brain\u2010derived neurotrophic factor (BDNF) signaling pathway mediates central sensitization through its involvement in spinal cord synaptic plasticity. Our group has previously found that electroacupuncture (EA) has a good analgesic effect on CSR. However, the central analgesic mechanism of EA for CSR is not yet clear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The rats were randomly divided into Blank group, Sham\u2010operated group, CSR group, and EA group. We prepared the CSR rat model using the fish wire extrusion method. The behavioral and mechanical pain thresholds of the rats in each group were measured 5 days after successful modeling and 7 days after the intervention. The first intervention was started 5 days after successful modeling, and the EA group was treated by acupuncture at the bilateral LI4 and LR3 points on the same side as one group, connected to a G6805\u2010I electroacupuncture apparatus with continuous waves at 1.5\u00a0Hz. The remaining groups were not subjected to EA intervention. The treatment was administered once a day for 7 consecutive days and then executed. We used WB, immunofluorescence, and qRT\u2010PCR to detect the expression of CaMKII\/CREB\/BDNF signaling pathway\u2010related factors in the synaptic of rat spinal cord in each group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>EA improved pain threshold and motor function in CSR rats, inhibited the expression of BDNF, P\u2010TrkB, CAMKII, and P\u2010CREB in spinal cord synapses, reduced the expression of pain factor c\u2010fos and postsynaptic membrane protein molecule neuroligin2, exerted a modulating effect on spinal cord synaptic plasticity in CSR rats, and suppressed the overactive synaptic efficacy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>EA mediates central sensitization and exerts analgesic effects on CSR by modulating spinal synaptic plasticity, which may be related to the inhibition of CaMKII\/CREB\/BDNF signaling pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.3177","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T10:09:09Z","timestamp":1691402949000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Electroacupuncture ameliorates pain in cervical spondylotic radiculopathy rat by inhibiting the CaMKII\/CREB\/BDNF signaling pathway and regulating spinal synaptic plasticity"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7316-970X","authenticated-orcid":false,"given":"Hong","family":"Su","sequence":"first","affiliation":[{"name":"The first school of clinical medicine, Guangxi of university of Chinese medicine Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of nursing The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Zhang","sequence":"additional","affiliation":[{"name":"The first school of clinical medicine, Guangxi of university of Chinese medicine Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengyong","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Acupuncture and Moxibustion The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"The first school of clinical medicine, Guangxi of university of Chinese medicine Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjun","family":"Guo","sequence":"additional","affiliation":[{"name":"The first school of clinical medicine, Guangxi of university of Chinese medicine Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiongxiao","family":"Wang","sequence":"additional","affiliation":[{"name":"The first school of clinical medicine, Guangxi of university of Chinese medicine Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caiyun","family":"Xie","sequence":"additional","affiliation":[{"name":"The first school of clinical medicine, Guangxi of university of Chinese medicine Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6231-4721","authenticated-orcid":false,"given":"Pu","family":"Yang","sequence":"additional","affiliation":[{"name":"The first school of clinical medicine, Guangxi of university of Chinese medicine Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/pm\/pnz017"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2016.68"},{"issue":"05","key":"e_1_2_6_4_1","first-page":"1154","article-title":"Effects of \"qi\u2010regulating\" electro\u2010acupuncture on JAK1 and STAT3 protein expression in local spinal cord tissues of CSR rats","volume":"36","author":"Chen S. 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