{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T05:29:20Z","timestamp":1788067760056,"version":"build-2784847793"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T00:00:00Z","timestamp":1689897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171464"],"award-info":[{"award-number":["82171464"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171180"],"award-info":[{"award-number":["82171180"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81901097"],"award-info":[{"award-number":["81901097"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010270","name":"Capital Health Research and Development of Special Fund","doi-asserted-by":"publisher","award":["2022\u20104\u20105025"],"award-info":[{"award-number":["2022\u20104\u20105025"]}],"id":[{"id":"10.13039\/501100010270","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Ferroptosis has recently been recognized as a new cause of ischemia reperfusion injury due to blood\u2013brain barrier (BBB) disruption followed by secondary iron\u2010loaded transferrin (TF) influx. As a novel and independent cell death pathway, ferroptosis was characterized by iron\u2010dependent lipid peroxidation, decline of GSH, GPX4, and shrinking mitochondria. Cottonseed oil (CSO), a liposoluble solvent, can alleviate ischemia stroke injuries and oxidative stress. However, the effect of CSO on ischemic stroke\u2013induced ferroptosis has not been explored. In this study, we investigated the effect of CSO on ferroptosis caused by cerebral ischemic injury in rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We conducted the subcutaneous injection of 1.3\u00a0mL\/kg CSO every other day for 3 weeks on rats with middle cerebral artery occlusion\u2013reperfusion (MCAO\u2010R) injury. We used Garcia Test, TTC staining, HE, Nissl and NeuN staining, Evans blue test,\n                      <jats:sup>68<\/jats:sup>\n                      Ga\u2010citrate PET, Western blot, immunofluorescence staining, Elisa kits, and transmission electron microscopy to detect the infarct volume, neural injuries, and ferroptosis\u2010related indexes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CSO treatment could significantly ameliorate MCAO\u2010R\u2010induced neurological dysfunction in a male rat model. Furthermore, it reduced infarct volume and neuronal injuries; protected BBB integrity; reduced the influx of iron ion, TF, and TF receptors; up\u2010regulated anti\u2010ferroptosis proteins (GPX4, xCT, HO1, FTH1), while down\u2010regulating ferroptosis\u2010related protein ACSL4; increased the activity of GSH and SOD; and decreased MDA and LPO levels. Mitochondrial destruction induced by ischemic stroke was also alleviated by CSO treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>CSO treatment can alleviate ischemic stroke injury via ferroptosis inhibition, which provides a new potential therapeutic mechanism for CSO neuroprotection against ischemic stroke.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.3179","type":"journal-article","created":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T23:40:05Z","timestamp":1689982805000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Cottonseed oil alleviates ischemic stroke injury by inhibiting ferroptosis"],"prefix":"10.1002","volume":"13","author":[{"given":"Miao","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Anesthesiology The First Medical Center of Chinese PLA General Hospital Beijing China"},{"name":"Department of Anesthesiology The First Affiliated Hospital Jinzhou Medical University Jinzhou Liaoning Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Beijing Tongren Hospital, Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingxiao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine The First Medical Center of Chinese PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The First Medical Center of Chinese PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Affiliated Hospital of Nantong University Nantong Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huikai","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The First Medical Center of Chinese PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Tangdu Hospital Air Force Military Medical University Xi'an Shaanxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahe","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine The First Medical Center of Chinese PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weidong","family":"Mi","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The First Medical Center of Chinese PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0982-9571","authenticated-orcid":false,"given":"Yulong","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The First Medical Center of Chinese PLA General Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464\u2010410X.2010.09689.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.03.032"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pez103"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2016.03.029"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11307\u2010016\u20100966\u20105"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.93999"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2016.09.006"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010019\u20100118\u20108"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2021.632354"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.042"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2239"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.103378"},{"key":"e_1_2_10_14_1","first-page":"159","volume-title":"Edible oils and fat products: Oils and oil seeds","author":"Jones L. 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