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Inflammation and immune imbalance have been identified as potential factors contributing to the occurrence and progression of MMD. However, the specific proteins and metabolites responsible for triggering this process are yet to be established. The purpose of this study is to identify differentially expressed proteins and metabolites in patients with MMD and perform Kyoto Encyclopedia of Genes and Genomes pathway integration analysis to pinpoint crucial proteins and metabolites involved in the disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed untargeted metabolomic and data\u2010independent acquisition proteomic analyses on the serum samples of individuals with MMD and healthy controls (HC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In patients with MMD versus HC, 24 proteins and 60 metabolites, including 21 anionic metabolites and 39 cationic metabolites, which were significantly different, were identified. In patients with MMD, several proteins involved in inflammation and immune metabolism, such as tubulin beta\u20106 and complement C4, were found to have significantly altered levels. Similarly, many metabolites involved in inflammation and immune metabolisms, such as dimethyl 4\u2010hydroxyisophthalate, beta\u2010nicotinamide mononucleotide, 2\u2010(3\u2010(4\u2010pyridyl)\u20101<jats:italic>H<\/jats:italic>\u20101,2,4\u2010triazol\u20105\u2010yl)pyridine, and PC (17:1\/18:2), were significantly altered. Intriguingly, these proteins and metabolites are involved in the progression of atherosclerosis through immune and inflammatory pathways, although some have never been reported in MMD. Moreover, integrated proteomics and metabolomics studies were conducted to determine shared pathways involving cholesterol metabolism, vitamin digestion, fat digestion, and absorption pathways of proteins and metabolites, which warrant further investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Significant increases in pro\u2010inflammatory and immunosuppressive abilities have been observed in patients with MMD, accompanied by significant reductions in anti\u2010inflammatory and immune regulation. Various metabolites and proteins implicated in these processes have been identified for the first time. These findings hold immense significance for comprehending the pathogenesis of MMD and for the development of future drug therapies.<\/jats:p><\/jats:sec>","DOI":"10.1002\/brb3.3328","type":"journal-article","created":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T11:14:19Z","timestamp":1699960459000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Proteomic and metabolomic characterizations of moyamoya disease patient sera"],"prefix":"10.1002","volume":"13","author":[{"given":"Qingbao","family":"Guo","sequence":"first","affiliation":[{"name":"Medical School of Chinese PLA  Beijing China"},{"name":"Department of Neurosurgery, The First Medical Centre Chinese PLA General Hospital  Beijing China"},{"name":"Department of Neurosurgery, The Fifth Medical Centre Chinese PLA General Hospital  Beijing 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