{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T19:27:16Z","timestamp":1773170836541,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Ischemic stroke is a disease in which cerebral blood flow is blocked due to various reasons, leading to ischemia, hypoxia, softening, and even necrosis of brain tissues. The level of cortisol is related to the occurrence and progression of ischemic stroke. However, the mechanism governing their interrelationship is still unclear. The main objective of this study was to identify and understand the molecular mechanism between cortisol and IS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The common cortisol\u2010related biological processes were screened by mutual verification of two data sets and the cortisol\u2010related hub biomarkers were identified. Modular analysis of protein interaction networks was performed, and the differential pathway analysis of individual genes was conducted by GSVA and GSEA. Drug and transcription factor regulatory networks of hub genes were excavated, and the diagnostic potential of hub genes was analyzed followed by the construction of a diagnostic model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>By screening the two data sets by GSVA, three biological processes with common differences were obtained. After variation analysis, four cortisol\u2010related hub biomarkers (CYP1B1, CDKN2B, MEN1, and USP8) were selected. Through the modular analysis of the protein\u2010protein interaction network and double verification of GSVA and GSEA, a series of potential molecular mechanisms of hub genes were discovered followed by a series of drug regulatory networks and transcription factor regulatory networks. The hub biomarkers were found to have a high diagnostic value by ROC; thus, a diagnostic model with high diagnostic efficiency was constructed. The diagnostic value was mutually confirmed in the two data sets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Four cortisol\u2010related hub biomarkers are identified in this study, which provides new ideas for the key changes of cortisol during the occurrence of IS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.3358","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T06:00:27Z","timestamp":1706508027000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification and validation of cortisol\u2010related hub biomarkers and the related pathogenesis of biomarkers in Ischemic Stroke"],"prefix":"10.1002","volume":"14","author":[{"given":"Jing\u2010Jing","family":"Wang","sequence":"first","affiliation":[{"name":"Neurology Department The First Affiliated Hospital of Zhengzhou University Zhengzhou China"},{"name":"Neurology Department People's Hospital of Luanchuan Luoyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1345-3722","authenticated-orcid":false,"given":"Fang\u2010Biao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Encephalopathy The First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou China"},{"name":"The First Clinical Medical College Henan University of Chinese Medicine Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sen","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Medical Records Zhengzhou University People's Hospital Henan Provincial People's Hospital Zhengzhou Henan People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2689-9897","authenticated-orcid":false,"given":"Yu\u2010Ming","family":"Xu","sequence":"additional","affiliation":[{"name":"Neurology Department The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin\u2010Zhi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Encephalopathy The First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou China"},{"name":"The First Clinical Medical College Henan University of Chinese Medicine Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2017.05.046"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ane.12847"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/cen.13802"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2021.101494"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/meta.2001.25592"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2016\u20100089"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415\u2010013\u20107231\u20105"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/19.2.185"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.22.558.309"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.802453"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201012\u201035"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2022.946593"},{"key":"e_1_2_8_14_1","first-page":"1","article-title":"The igraph software package for complex network research","author":"Csardi G.","year":"2006","journal-title":"InterJournal, complex systems 1695"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1009794"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1084"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1013"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201014\u20107"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000004597"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.02.028"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.635021"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/cen.13796"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.4760"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2018.03.018"},{"key":"e_1_2_8_25_1","unstructured":"Morgan M. 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