{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T20:36:20Z","timestamp":1774989380672,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T00:00:00Z","timestamp":1710201600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To distinguish between the genuine cellular impact of the ischemic cascade by leukocytes and unspecific effects of edema and humoral components, two knock\u2010in mouse lines were utilized. Mouse lines Y731F and Y685F possess point mutations in VE\u2010cadherin, which lead to a selective inhibition of transendothelial leukocyte migration or impaired vascular permeability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ischemic stroke was induced by a model of middle cerebral artery occlusion. Analysis contained structural outcomes (infarct volume and extent of brain edema), functional outcomes (survival analysis, rotarod test, and neuroscore), and the extent and spatial distribution of leukocyte migration (heatmaps and fluorescence\u2010activated cell sorting (FACS) analysis).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Inhibition of transendothelial leukocyte migration as in Y731F mice leads to smaller infarct volumes (52.33\u00a0\u00b1\u00a04719 vs. 70.43\u00a0\u00b1\u00a06483\u00a0mm<jats:sup>3<\/jats:sup>, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.0252) and improved motor skills (rotarod test: 85.52\u00a0\u00b1\u00a013.24\u00a0s vs. 43.06\u00a0\u00b1\u00a015.32\u00a0s, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.0285). An impaired vascular permeability as in Y685F mice showed no effect on structural or functional outcomes. Both VE\u2010cadherin mutations did not influence the total immune cell count or spatial distribution in ischemic brain parenchyma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Selective inhibition of transendothelial leukocyte migration by VE\u2010cadherin mutation after ischemic stroke in a mouse model leads to smaller infarct volumes and improved motor skills.<\/jats:p><\/jats:sec>","DOI":"10.1002\/brb3.3449","type":"journal-article","created":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T07:09:37Z","timestamp":1710227377000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Inhibition of leukocyte migration after ischemic stroke by VE\u2010cadherin mutation in a mouse model leads to reduced infarct volumes and improved motor skills"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7097-0335","authenticated-orcid":false,"given":"Mailin Hannah 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