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Previous work from our laboratory revealed that in the soleus and gluteus minimus muscles of mice overexpressing <jats:styled-content style=\"fixed-case\">PLN<\/jats:styled-content> (<jats:italic>Pln<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:sup>OE<\/jats:sup><\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">SERCA<\/jats:styled-content> function was impaired, dynamin 2 (3\u20135 fold) and <jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content> (7\u20139 fold) were upregulated, and features of human centronuclear myopathy (<jats:styled-content style=\"fixed-case\">CNM<\/jats:styled-content>) were observed. Here, we performed structural and functional experiments to evaluate whether the diaphragm muscles of the <jats:italic>Pln<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:sup>OE<\/jats:sup><\/jats:styled-content> mouse would exhibit <jats:styled-content style=\"fixed-case\">CNM<\/jats:styled-content> pathology and muscle weakness.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Diaphragm muscles from <jats:italic>Pln<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:sup>OE<\/jats:sup><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> mice were subjected to histological\/histochemical\/immunofluorescent staining, Ca<jats:sup>2+<\/jats:sup>\u2010<jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>ase and Ca<jats:sup>2+<\/jats:sup> uptake assays, Western blotting, and <jats:italic>in\u00a0vitro<\/jats:italic> electrical stimulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results demonstrate that <jats:styled-content style=\"fixed-case\">PLN<\/jats:styled-content> overexpression reduced <jats:styled-content style=\"fixed-case\">SERCA<\/jats:styled-content>'s apparent affinity for Ca<jats:sup>2+<\/jats:sup> but did not reduce maximal <jats:styled-content style=\"fixed-case\">SERCA<\/jats:styled-content> activity or rates of Ca<jats:sup>2+<\/jats:sup> uptake. <jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content> was upregulated 2.5\u2010fold, whereas no changes in dynamin 2 expression were found. With respect to <jats:styled-content style=\"fixed-case\">CNM<\/jats:styled-content>, we did not observe type I fiber predominance, central nuclei, or central aggregation of oxidative activity in diaphragm, although type I fiber hypotrophy was present. Furthermore, <jats:italic>in\u00a0vitro<\/jats:italic> contractility assessment of <jats:italic>Pln<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:sup>OE<\/jats:sup><\/jats:styled-content> diaphragm strips revealed no reductions in force\u2010generating capacity, maximal rates of relaxation or force development, but did indicate that \u00bd relaxation time was prolonged.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Therefore, the effects of <jats:styled-content style=\"fixed-case\">PLN<\/jats:styled-content> overexpression on skeletal muscle phenotype differ between diaphragm and the postural soleus and gluteus minimus muscles. Our findings here point to differences in <jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content> expression and type I fiber distribution as potential contributing factors.<\/jats:p><\/jats:sec>","DOI":"10.1002\/brb3.470","type":"journal-article","created":{"date-parts":[[2016,4,22]],"date-time":"2016-04-22T22:23:23Z","timestamp":1461363803000},"source":"Crossref","is-referenced-by-count":21,"title":["Diaphragm assessment in mice overexpressing phospholamban in slow\u2010twitch type <scp>I<\/scp> muscle fibers"],"prefix":"10.1002","volume":"6","author":[{"given":"Val Andrew","family":"Fajardo","sequence":"first","affiliation":[{"name":"Department of Kinesiology University of Waterloo Waterloo ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian Curtis","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Kinesiology University of Waterloo Waterloo ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Bombardier","sequence":"additional","affiliation":[{"name":"Department of Kinesiology University of Waterloo Waterloo ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paige J.","family":"Chambers","sequence":"additional","affiliation":[{"name":"Department of Kinesiology University of Waterloo Waterloo ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joe","family":"Quadrilatero","sequence":"additional","affiliation":[{"name":"Department of Kinesiology University of Waterloo Waterloo ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allan Russell","family":"Tupling","sequence":"additional","affiliation":[{"name":"Department of Kinesiology University of Waterloo Waterloo ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C200269200"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1657"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035273"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2013.04.019"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.212498399"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032632"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.16.2499"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3003.1999.13e20.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.01.054"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI71206"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/386855a0"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01440.2006"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.31.21908"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084304"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.020859"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI36277"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.446161"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00495403"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2014.00339"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-3-26"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2013.269373"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI46267"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00488.2010"},{"key":"e_1_2_7_25_1","first-page":"184","article-title":"Density and composition of mammalian muscle","volume":"9","author":"Mendez J. 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