{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:02:59Z","timestamp":1772118179378,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,19]],"date-time":"2025-01-19T00:00:00Z","timestamp":1737244800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014717","name":"National Outstanding Youth Science Fund Project of National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC"],"award-info":[{"award-number":["NSFC"]}],"id":[{"id":"10.13039\/100014717","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014717","name":"National Outstanding Youth Science Fund Project of National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101588"],"award-info":[{"award-number":["82101588"]}],"id":[{"id":"10.13039\/100014717","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Post\u2010traumatic stress disorder (PTSD) is a complex psychiatric condition that emerges following exposure to trauma and significantly affects daily functioning. Current research is focused on identifying effective treatments for PTSD. Advances in bioinformatics provide opportunities to elucidate the underlying mechanisms of PTSD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>RNA sequencing (RNA\u2010seq) datasets were retrieved from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified using GEO2R. Weighted gene co\u2010expression network analysis (WGCNA) was employed to examine gene correlation patterns. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed for functional annotation and enrichment analysis, respectively. The MCODE plugin in Cytoscape software was utilized to analyze the protein\u2013protein interaction (PPI) network. Anxiety and depression in a mice stress model were assessed using the open\u2010field test (OFT), elevated plus maze test (EPMT), and forced swimming test (FST). Real\u2010time quantitative PCR (qRT\u2010PCR) was conducted to validate key genes in stress\u2010exposed models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 157 common upregulated DEGs and 53 common downregulated DEGs were identified in the amygdala (AMY) and the hippocampus (HIP). Notably enriched pathways included neuroactive ligand\u2010receptor interaction, mechanistic target of rapamycin (mTOR) signaling pathway, nicotine addiction, and dopaminergic synapse. The PPI network identified four hub genes, with key pathways associated with nicotine addiction and dopaminergic synapse. qRT\u2010PCR validation confirmed that the expression trends of these four genes were consistent with microarray data. Behavioral tests (OFT, EMPT, and FST) revealed significant changes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study utilized bioinformatics and in vitro experiments to identify genes and pathways potentially crucial for PTSD development. Key genes were validated in a mouse model, providing insights into potential target genes for PTSD treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.70243","type":"journal-article","created":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T02:12:46Z","timestamp":1737339166000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification of Critical Signature in Post\u2010Traumatic Stress Disorder Using Bioinformatics Analysis and in Vitro Analyses"],"prefix":"10.1002","volume":"15","author":[{"given":"Lifen","family":"Liu","sequence":"first","affiliation":[{"name":"School of Psychology Shandong Second Medical University Weifang Shandong People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Psychological Counseling Center Weifang Mental Health Center, Weian Road, Weifang, Shandong  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Li","sequence":"additional","affiliation":[{"name":"School of Psychology Shandong Second Medical University Weifang Shandong People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Teng","sequence":"additional","affiliation":[{"name":"Faculty of Psychology Southwest University, Chongqing  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuyang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Bioscience and Technology Shandong Second Medical University Weifang Shandong People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinhong","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Extended Education Shandong Second Medical University Weifang Shandong People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changjiang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Psychology Shandong Second Medical University Weifang Shandong People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyu","family":"Hu","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Mental Health, Institute of Psychology Chinese Academy of Sciences Beijing People's Republic of China"},{"name":"Department of Psychology University of Chinese Academy of Sciences Beijing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7496-7685","authenticated-orcid":false,"given":"Lin","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Psychology Shandong Second Medical University Weifang Shandong People's Republic of China"},{"name":"Management Committee of Shanting Economic Development Zone Zaozhuang Shandong People's Republic of China"},{"name":"Department of Neurosurgery Shanting District People's Hospital Zaozhuang Shandong People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,19]]},"reference":[{"key":"e_1_2_12_2_1","unstructured":"A.P. 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