{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T17:49:04Z","timestamp":1784742544738,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000381","name":"Multiple Sclerosis Society","doi-asserted-by":"publisher","award":["1038154"],"award-info":[{"award-number":["1038154"]}],"id":[{"id":"10.13039\/501100000381","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Brain and Behavior"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>The common marmoset is a small nonhuman primate that has emerged as a valuable animal model in neuroscience research. Accurate analysis of brain tissue is crucial to understand marmoset neurophysiology and to model neurodegenerative diseases. Many studies to date have complemented magnetic resonance imaging (MRI) with histochemical staining rather than immunofluorescent labeling, which can generate more informative and higher resolution images. There is a need for high\u2010throughput immunolabeling and imaging methodologies to generate resources for the burgeoning marmoset field, particularly brain histology atlases to display the organization of different cell types and other structures.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Findings<\/jats:title>\n                    <jats:p>Here, we have characterized a set of marmoset\u2010compatible fluorescent dyes and antibodies that label myelin, axons, dendrites, and the iron\u2010storage protein ferritin, and developed a batch\u2010style multiplex immunohistochemistry protocol to uniformly process large numbers of tissue slides for multiple cell\u2010type specific markers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We provide a practical guide for researchers interested in harnessing the potential of marmoset models to advance understanding of brain structure, function, and pathophysiology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/brb3.70308","type":"journal-article","created":{"date-parts":[[2025,4,5]],"date-time":"2025-04-05T06:19:34Z","timestamp":1743833974000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Multiplex Immunofluorescent Batch Labeling of Marmoset Brain Sections"],"prefix":"10.1002","volume":"15","author":[{"given":"Daryan","family":"Chitsaz","sequence":"first","affiliation":[{"name":"Integrated Program in Neuroscience McGill University  Montr\u00e9al Quebec Canada"},{"name":"Department of Neurology &amp; Neurosurgery, Montreal Neurological Institute\u2010Hospital McGill University  Montr\u00e9al Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher D.","family":"Rowley","sequence":"additional","affiliation":[{"name":"Department of Physics &amp; Astronomy McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6951-0664","authenticated-orcid":false,"given":"Nonthu\u00e9 A.","family":"Uccelli","sequence":"additional","affiliation":[{"name":"Department of Neurology &amp; Neurosurgery, Montreal Neurological Institute\u2010Hospital McGill University  Montr\u00e9al Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Lefebvre","sequence":"additional","affiliation":[{"name":"Cognitive Neuroscience Unit, Montreal Neurological Institute\u2010Hospital McGill University  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea I.","family":"Krahn","sequence":"additional","affiliation":[{"name":"The Neuro's Early Drug Discovery Unit (EDDU) McGill University  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang E.","family":"Reintsch","sequence":"additional","affiliation":[{"name":"The Neuro's Early Drug Discovery Unit (EDDU) McGill University  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Durcan","sequence":"additional","affiliation":[{"name":"Integrated Program in Neuroscience McGill University  Montr\u00e9al Quebec Canada"},{"name":"Department of Neurology &amp; Neurosurgery, Montreal Neurological Institute\u2010Hospital McGill University  Montr\u00e9al Quebec Canada"},{"name":"The Neuro's Early Drug Discovery Unit (EDDU) McGill University  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine L.","family":"Tardif","sequence":"additional","affiliation":[{"name":"Integrated Program in Neuroscience McGill University  Montr\u00e9al Quebec Canada"},{"name":"Department of Neurology &amp; Neurosurgery, Montreal Neurological Institute\u2010Hospital McGill University  Montr\u00e9al Quebec Canada"},{"name":"Department of Biomedical Engineering, Faculty of Medicine and Health Sciences McGill University  Montr\u00e9al Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4454-5080","authenticated-orcid":false,"given":"Timothy E.","family":"Kennedy","sequence":"additional","affiliation":[{"name":"Integrated Program in Neuroscience McGill University  Montr\u00e9al Quebec Canada"},{"name":"Department of Neurology &amp; Neurosurgery, Montreal Neurological Institute\u2010Hospital McGill University  Montr\u00e9al Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.11.4685"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2011.07.002"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhy263"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-021-01145-2"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911177107"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/01926233221095449"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41597-023-02121-2"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep36410"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-8935-5_26"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586-023-02566-w"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2023.102256"},{"key":"e_1_2_9_13_1","volume-title":"A Novel Myelin Labeling Technique for Fluorescence Microscopy of Brain Sections","author":"Kilgore J.","year":"2004"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2020125118"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7MT00012J"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.18.7195"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.30498"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.40042"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14858-0"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2020.117625"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24023"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.03.018"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2012.06.015"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-neuro-030520-101844"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23137047"},{"key":"e_1_2_9_26_1","unstructured":"Paxinos G. 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